| Author | Serra, Magda F. | |
| Author | Cotias, Amanda C. | |
| Author | Pimentel, Andreza S. | |
| Author | Arantes, Ana Carolina S. de | |
| Author | Pires, Ana Lucia A. | |
| Author | Lanzetti, Manuella | |
| Author | Hickmann, Jandir M. | |
| Author | Barreto, Emiliano | |
| Author | Carvalho, Vinicius S. | |
| Author | Silva, Patrícia M. R. e | |
| Author | Cordeiro, Renato S. B. | |
| Author | Martins, Marco Aurélio | |
| Access date | 2022-10-21T12:14:09Z | |
| Available date | 2022-10-21T12:14:09Z | |
| Document date | 2022 | |
| Citation | SERRA, Magda F. et al. Gold Nanoparticles Inhibit Steroid-Insensitive Asthma in Mice Preserving Histone Deacetylase 2 and NRF2 Pathways. Antioxidants, v. 11, 1659, p. 1 - 21, Aug. 2022. | en_US |
| ISSN | 2076-3921 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55229 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Nanopartícula de ouro | en_US |
| Subject in Portuguese | Asma insensível a esteroides | en_US |
| Subject in Portuguese | Inflamação pulmonar | en_US |
| Subject in Portuguese | NRF2 | en_US |
| Subject in Portuguese | HDAC2 | en_US |
| Subject in Portuguese | Desequilíbrio Oxidativo | en_US |
| Title | Gold Nanoparticles Inhibit Steroid-Insensitive Asthma in Mice Preserving Histone Deacetylase 2 and NRF2 Pathways | en_US |
| Type | Article | |
| DOI | 10.3390/ antiox11091659 | |
| Abstract | Background: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in
experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study
assessed the effectiveness of nebulized AuNPs in a murine model of glucocorticoid (GC)-resistant
asthma. Methods: A/J mice were sensitized and subjected to intranasal instillations of ovalbumin
(OVA) once a week for nine weeks. Two weeks after starting allergen stimulations, mice were
subjected to Budesonide or AuNP nebulization 1 h before stimuli. Analyses were carried out
24 h after the last provocation. Results: We found that mice challenged with OVA had airway
hyperreactivity, eosinophil, and neutrophil infiltrates in the lung, concomitantly with peribronchiolar
fibrosis, mucus production, and pro-inflammatory cytokine generation compared to sham-challenged
mice. These changes were inhibited in mice treated with AuNPs, but not Budesonide. In the GCresistant
asthmatic mice, oxidative stress was established, marked by a reduction in nuclear factor
erythroid 2-related factor 2 (NRF2) levels and catalase activity, accompanied by elevated values of
thiobarbituric acid reactive substances (TBARS), phosphoinositide 3-kinases (PI3K ) expression, as
well as a reduction in the nuclear expression of histone deacetylase 2 (HDAC2) in the lung tissue, all
of which sensitive to AuNPs but not Budesonide treatment. Conclusion: These findings suggest that
AuNPs can improve GC-insensitive asthma by preserving HDAC2 and NRF2. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Instituto de Física. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade federal de Alagoas. Laboratório de Biologia Celular. Maceió, AL, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Gold nanoparticle | en_US |
| Subject | Steroid-insensitive asthma | en_US |
| Subject | Lung inflammation | en_US |
| Subject | NRF2 | en_US |
| Subject | HDAC2 | en_US |
| Subject | Oxidative Imbalance | en_US |
