| Author | Carvalho, Renata Faria de | |
| Author | Quintaes, Lucas de Siqueira Penna | |
| Author | Su, Thaís de Cássia de Souza | |
| Author | Kobayashi, Leticia Mitiko | |
| Author | Nogueira, Ana Cristina Martins de Almeida | |
| Access date | 2022-10-26T14:54:06Z | |
| Available date | 2022-10-26T14:54:06Z | |
| Document date | 2022 | |
| Citation | CARVALHO, Renata faria de et al. Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. Plos One, v. 17, n. 5, e0267653, p. 1 - 14, May 2022. | en_US |
| ISSN | 1932-6203 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55350 | |
| Language | eng | en_US |
| Publisher | Public Library of Science | en_US |
| Rights | open access | |
| Subject in Portuguese | Viabilidade da vacina da Dengue | en_US |
| Subject in Portuguese | Infectar diferentes linhagens de células humanas | en_US |
| Subject in Portuguese | Teste de potência alternativo | en_US |
| Subject in Portuguese | Uso de células HEK293T | en_US |
| Subject in Portuguese | Comunicação curta | en_US |
| Title | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells | en_US |
| Type | Article | |
| DOI | 10.1371/ journal.pone.0267653 | |
| Abstract | Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four
distinct, but close related, circulating serotypes. Dengue disease is of great importance for
global public health, with vaccination being its main prophylactic measure. However, there
is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this
study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial
dengue vaccine and test the feasibility of this approach in the development of a
potency assay with human cell lines, as a methodological alternative to the golden standard
potency assay with VERO cells. In this context, we used a batch of the commercial vaccine
Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine
virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal
microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined
by immunocolorimetric assay using monoclonal antibodies specific for each serotype.
The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine,
as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to
the one observed with VERO cells. Moreover, it was possible to determine the infectivity
and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing
over the others. Thus, the human cell line HEK293T provides a potential candidate to be
used in assays to determine potency and identity of tetravalent dengue vaccines. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Vacinas Virais. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância Sanitária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Vacinas Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Vacinas Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Vacinas Virais. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância Sanitária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Feasibility of dengue vaccine | en_US |
| Subject | Infect different human cell lines | en_US |
| Subject | Alternative potency test | en_US |
| Subject | Using HEK293T cells | en_US |
| Subject | Short communication | en_US |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
