Author | Caldeira, Roberta Lima | |
Author | Teodoro, Tatiana Maria | |
Author | Gomes, Maria Flávia Belfort | |
Author | Carvalho, Omar dos Santos | |
Access date | 2012-09-26T17:18:44Z | |
Available date | 2012-09-26T17:18:44Z | |
Document date | 2010 | |
Citation | CALDEIRA, Roberta Lima et al. Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil. Mem Inst Oswaldo Cruz. 2010 Jul;105(4):485-7. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/5545 | |
Language | eng | pt_BR |
xmlui.metadata.dc.relation.ispartofseries | Mémorias do Insituto Oswaldo Cruz | pt_BR |
xmlui.metadata.dc.relation.isbasedon | Caldeira RL, Teodoro TM, Gomes MF, Carvalho Odos S. . Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil. Mem Inst Oswaldo Cruz. 2010 Jul;105(4):485-7. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Biomphalaria cousin | pt_BR |
Subject in Portuguese | Biomphalaria amazonica | pt_BR |
Subject in Portuguese | PCR-RFLP | pt_BR |
Subject in Portuguese | Morphological characters | pt_BR |
Title | Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil | pt_BR |
Type | Article | pt_BR |
Abstract | Specific genetic profiles of Brazilian Biomphalaria species were previously standardized by molecular taxonomy through the analysis of restriction fragments, which were generated by digesting the internal transcribed spacer re¬gion of rDNA with the DdeI endonuclease. Biomphalaria amazonica displayed three distinct profiles. To investigate these distinct profiles, the same molecular technique, polymerase chain reaction and restriction fragment length polymorphism, was used with different endonucleases. In addition, morphological data were also used to compare B. amazonica specimens that were collected from Brazil, Colombia and Bolivia. The morphological characters of Boliv¬ian molluscs were similar to B. amazonica, displayed a molecular profile of five restriction fragments and morpho¬logical data, whereas the Colombian mollusc population showed morphological characters similar to Biomphalaria cousini and a molecular profile of three restriction fragments, similar to B. cousini. The Brazilian specimens showed the B. amazonica and B. cousini molecular profiles as well as a third profile, which resembled a combination of the Colombian and Bolivian molecular profiles | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | pt_BR |