| Author | Sousa, Ingrid S. | |
| Author | Mello, Thaís P. | |
| Author | Pereira, Elaine P. | |
| Author | Granato, Marcela Q. | |
| Author | Alviano, Celuta S. | |
| Author | Santos, André L. S. | |
| Author | Kneipp, Lucimar F. | |
| Access date | 2022-11-06T19:50:11Z | |
| Available date | 2022-11-06T19:50:11Z | |
| Document date | 2022 | |
| Citation | SOUSA, Ingrid S. et al. Biofilm Formation by Chromoblastomycosis Fungi Fonsecaea pedrosoi and Phialophora verrucosa: Involvement with Antifungal Resistance. Journal of Fungi, v. 9, 963, p. 1 - 18, Sept. 2022. | en_US |
| ISSN | 2309-608X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55480 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Biofilme | en_US |
| Subject in Portuguese | Virulência | en_US |
| Subject in Portuguese | Resistência antifúngica | en_US |
| Subject in Portuguese | Fungos demáceos | en_US |
| Subject in Portuguese | Bombas de efluxo | en_US |
| Title | Biofilm formation by Chromoblastomycosis fungi Fonsecaea pedrosoi and Phialophora verrucosa: involvement with antifungal resistance | en_US |
| Type | Article | |
| DOI | 10.3390/ jof8090963 | |
| Abstract | Patients with chromoblastomycosis (CBM) suffer chronic tissue lesions that are hard to treat.
Considering that biofilm is the main growth lifestyle of several pathogens and it is involved with both
virulence and resistance to antimicrobial drugs, we have investigated the ability of CBM fungi to produce
this complex, organized and multicellular structure. Fonsecaea pedrosoi and Phialophora verrucosa
conidial cells were able to adhere on a polystyrene abiotic substrate, differentiate into hyphae and
produce a robust viable biomass containing extracellular matrix. Confocal laser scanning microscopy
(CLSM) and scanning electron microscopy (SEM) showed the tridimensional architecture of the
mature biofilms, revealing a dense network of interconnected hyphae, inner channels and amorphous
extracellular polymeric material. Interestingly, the co-culture of each fungus with THP-1 macrophage
cells, used as a biotic substrate, induced the formation of a mycelial trap covering and damaging the
macrophages. In addition, the biofilm-forming cells of F. pedrosoi and P. verrucosa were more resistant
to the conventional antifungal drugs than the planktonic-growing conidial cells. The efflux pump
activities of P. verrucosa and F. pedrosoi biofilms were significantly higher than those measured in
conidia. Taken together, the data pointed out the biofilm formation by CBM fungi and brought up a
discussion of the relevance of studies about their antifungal resistance mechanisms. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes (IMPG). Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes (LEAMER),. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes (IMPG). Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes (LEAMER),. Rio de Janeiro, RJ, Brasil / Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). Rede Micologia RJ. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil / Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). Rede Micologia RJ. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Biofilm | en_US |
| Subject | Antifungal resistance | en_US |
| Subject | Dematiaceous fungi | en_US |
| Subject | Efflux pumps | en_US |
| Subject | Virulence | en_US |
