| Author | Paiva, Luciene Pimenta de | |
| Author | Reis, Jordana Grazziela Alves Coelho Dos | |
| Author | Trindade, Bruno Caetano | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Araújo, Márcio Sobreira Silva | |
| Author | Gonçalves, Juan Jonathan | |
| Author | Nogueira, Ana Caroline | |
| Author | Martins, Júlia Pereira | |
| Author | Ribeiro, Ágata Lopes | |
| Author | Starling, Ana Lucia | |
| Author | Alcântara, Luiz Carlos Júnior | |
| Author | Ribeiro, Maísa Aparecida | |
| Author | Proietti, Anna Bárbara de Freitas Carneiro | |
| Author | Sabino, Ester Cerdeira | |
| Author | Bicalho, Kelly Alves | |
| Author | Carvalho, Andréa Teixeira | |
| Author | Martins Filho, Olindo Assis | |
| Access date | 2022-11-17T17:43:10Z | |
| Available date | 2022-11-17T17:43:10Z | |
| Document date | 2022 | |
| Citation | PAIVA, Luciene Pimenta de et al. A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection. Frontiers in Immunology, v. 13, 795815, p. 1-15, Apr. 2022. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55682 | |
| Language | eng | en_US |
| Publisher | Frontiers Research Foundation | en_US |
| Rights | open access | |
| Title | A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection | en_US |
| Type | Article | |
| DOI | 10.3389/fimmu.2022.795815 | |
| Abstract | In the present work, we developed and evaluated the performance of a new flow cytometry-based single platform, referred to as "FC-Duplex IgG1 (HTLV-1/2)", for universal and differential serodiagnosis of HTLV-1/2 infection. The proposed technology employs a system for detection of IgG1 antibodies in a single competitive immunofluorescence platform by flow cytometry using fluorescently labeled MT-2/MoT cell line mix coupled to a highly sensitive development system (Biotin/Streptavidin/Phycoerythrin). The stability of fluorescent labeling and the antigenicity of MT-2 and MoT cell lines were confirmed upon storage at -20°C for 2, 6, and 12 months. The anti-HTLV-1/2 IgG1 reactivity, expressed as percentage of positive fluorescent cells (PPFC), was evaluated for each target antigen along the titration curve of test serum samples (1:32 to 1:4,096). Upon selection of target cell line and serum dilutions with higher segregation score between groups, the performance of "FIX" and "FIX & PERM" protocols was evaluated. The "FIX" protocol presented excellent performance indices (Se = 92%/Sp = 94%/AUC = 0.96; Se = 96%/Sp = 100%/AUC = 0.99) for the universal (HTLV-1/2 vs. NI) and differential (HTLV-1 vs. HTLV-2) diagnosis of HTLV-1 infection, respectively. Optimization of the "FIX" protocol using the principle of synchronous and asynchronous pairwise analysis further improved the performance of "FC-Duplex IgG1 (HTLV-1/2)", using the "FIX" protocol for differential diagnosis of HTLV-1 and HTLV-2 infections (Se = 100%/Sp = 100%/AUC = 1.00). In conclusion, the "FC-Duplex IgG1 (HTLV-1/2)" method represents an innovation in the biotechnology segment with the potential to compose a serological kit for differential diagnosis of HTLV-1/2 infection for reference laboratories and blood centers. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Estado de Minas Gerais. Fundação Centro de Hematologia e Hemoterapia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Estado de Minas Gerais. Fundação Centro de Hematologia e Hemoterapia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Subject | HTLV | en_US |
| Subject | HTLV-1/2 | en_US |
| Subject | Competitive assay | en_US |
| Subject | Flow cytometry | en_US |
| Subject | Universal and differential diagnosis | en_US |
