| Author | Chacon, Daisy Sotero | |
| Author | Santos, Marlon Dias Mariano | |
| Author | Bonilauri, Bernardo | |
| Author | Vilasboa, Johnatan | |
| Author | Costa, Cibele Tesser da | |
| Author | Silva, Ivanice Bezerra da | |
| Author | Torres, Taffarel de Melo | |
| Author | Araújo, Thiago Ferreira de | |
| Author | Roque, Alan de Araújo | |
| Author | Pilon, Alan Cesar | |
| Author | Selegatto, Denise Medeiros | |
| Author | Freire, Rafael Teixeira | |
| Author | Reginaldo, Fernanda Priscila Santos | |
| Author | Voigt, Eduardo Luiz | |
| Author | Zuanazzi, José Angelo Silveira | |
| Author | Scortecci, Kátia Castanho | |
| Author | Cavalheiro, Alberto José | |
| Author | Lopes, Norberto Peporine | |
| Author | Ferreira, Leandro De Santis | |
| Author | Santos, Leandro Vieira dos | |
| Author | Fontes, Wagner | |
| Author | Sousa, Marcelo Valle de | |
| Author | Carvalho, Paulo Costa | |
| Author | Fett-Neto, Arthur Germano | |
| Author | Giordani, Raquel Brandt | |
| Access date | 2022-11-29T15:11:36Z | |
| Available date | 2022-11-29T15:11:36Z | |
| Document date | 2022 | |
| Citation | CHACON, Daisy Sotero et al. Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant. Front. Plant Sci, v. 13, n. 947558, p. 1–22, 2022. | en_US |
| ISSN | 1664-462X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55824 | |
| Language | por | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Erythrina velutin | en_US |
| Subject in Portuguese | Caatinga | en_US |
| Title | Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina willd., a brazilian semiarid native woody plant | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fpls.2022.947558 | |
| Abstract | Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh condition of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the dierentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Farmácia. Natal, RN, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
| Affilliation | Stanford Cardiovascular Institute. Stanford University School of Medicine. Stanford, CA, United States. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia e Departamento de Botânica. Laboratório de Fisiologia Vegetal. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia e Departamento de Botânica. Laboratório de Fisiologia Vegetal. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Farmácia. Natal, RN, Brasil. | en_US |
| Affilliation | Universidade Federal Rural do Semi-Árido. Núcleo de Bioinformática, Bioestatística e Biologia Computacional. Mossoró, RN, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Farmácia. Natal, RN, Brasil. | en_US |
| Affilliation | Herbário do Parque das Dunas. Instituto de Desenvolvimento Sustentável e Meio Ambiente. Natal, RN, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Biomolecular. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Zimmermann Group. European Molecular Biology Laboratory. Structural and Computational Biology Unit. Heidelberg, Germany. | en_US |
| Affilliation | Signal and Information Processing for Sensing Systems. Institute for Bioengineering of Catalonia. Barcelona Institute of Science and Technology. Barcelona, Spain. | en_US |
| Affilliation | Institute of Biology. Leiden University. Leiden, Netherlands. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Biociências. Departamento de Biologia Celular e Genética. Natal, RN, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Laboratório de Farmacognosia. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Centro de Biociências. Departamento de Biologia Celular e Genética. Natal, RN, Brasil. | en_US |
| Affilliation | Universidade Estadual de São Paulo. Instituto de Química. Araraquara, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Biomolecular. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Farmácia. Natal, RN, Brasil. | en_US |
| Affilliation | Universidade de Campinas. Instituto de Biologia. Programa de Pós-Graduação em Genética e Biologia Molecular. Campinas, SP, Brasil. | en_US |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Bioquímica e Química de Proteínas. Brasília, DF, Brasil. | en_US |
| Affilliation | Universidade de Brasília. Departamento de Biologia Celular. Laboratório de Bioquímica e Química de Proteínas. Brasília, DF, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia e Departamento de Botânica. Laboratório de Fisiologia Vegetal. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Farmácia. Natal, RN, Brasil. | en_US |
| Subject | Flavonoids | en_US |
| Subject | Transcriptome | en_US |
| Subject | Phenolic Compounds | en_US |
| Subject | Phytochemicals | en_US |
| Subject in Spanish | Flavonoides | en_US |
| Subject in Spanish | Compuestos Fenólicos | en_US |
| Subject in Spanish | Fitoquímicos | en_US |
| Subject in French | Flavonoïdes | en_US |
| Subject in French | Composés Phénoliques | en_US |
| Subject in French | Composés phytochimiques | en_US |
| DeCS | Erythrina | en_US |
| DeCS | Flavonoides | en_US |
| DeCS | Transcriptoma | en_US |
| DeCS | Compostos Fenólicos | en_US |
| DeCS | Compostos Fitoquímicos | en_US |
