| Author | Ostolin, Thais Lopes Valentim Di Paschoale | |
| Author | Gusmão, Miriã Rodrigues | |
| Author | Mathias, Fernando Augusto Siqueira | |
| Author | Cardoso, Jamille Mirelle de Oliveira | |
| Author | Roatt, Bruno Mendes | |
| Author | Aguiar-Soares, Rodrigo Dian de Oliveira | |
| Author | Ruiz, Jeronimo Conceição | |
| Author | Resende, Daniela de Melo | |
| Author | Brito, Rory Cristiane Fortes de | |
| Author | Reis, Alexandre Barbosa | |
| Access date | 2022-12-12T17:04:55Z | |
| Available date | 2022-12-12T17:04:55Z | |
| Document date | 2021 | |
| Citation | OSTOLIN, Thais Lopes Valentim Di Paschoale et al. A chimeric vaccine combined with adjuvant system induces immunogenicity and protection against visceral leishmaniasis in BALB/c mice. Vaccine, v. 39, n. 20, p. 2755-2763, 2021. doi: 10.1016/j.vaccine.2021.04.004. | en_US |
| ISSN | 0264-410X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55921 | |
| Language | eng | en_US |
| Publisher | Elsevier Science | en_US |
| Rights | open access | en_US |
| Title | A chimeric vaccine combined with adjuvant system induces immunogenicity and protection against visceral leishmaniasis in BALB/c mice | en_US |
| Type | Article | en_US |
| Abstract | In Brazil, canine visceral leishmaniasis is an important public health problem due to its alarming growth. The high prevalence of infected dogs reinforces the need for a vaccine for use in prophylactic vaccination campaigns. In the present study, we evaluate the immunogenicity and protection of the best dose of Chimera A selected through the screening of cytokines production important in disease. BALB/c mice were vaccinated subcutaneously with three doses and challenged intravenously with 1 × 107L. infantum promastigotes. Spleen samples were collected to assess the intracellular cytokine profile production, T cell proliferation and parasite load. At first, three different doses of Chimera A (5 μg, 10 μg and 20 μg) were evaluated through the production of IFN-γ and IL-10 cytokines. Since the dose of 20 μg showed the best results, it was chosen to continue the study. Secondarily, Chimera A at dose of 20 μg was formulated with Saponin plus Monophosphoryl lipid A. Vaccination with Chimera A alone and formulated with SM adjuvant system was able to increase the percentage of the proliferation of specific T lymphocytes and stimulated a Th1 response with increased levels of IFN-γ, TNF-α and IL-2, and decreased of IL-4 and IL-10. The vaccine efficacy through real-time PCR demonstrated a reduction in the splenic parasite load in animals that received Chimera A formulated with the SM adjuvant system (92%). Additionally, we observed increased levels of nitric oxide in stimulated-culture supernatants. The Chimera A formulated with the SM adjuvant system was potentially immunogenic, being able to induce immunoprotective mechanisms and reduce parasite load. Therefore, the use of T-cell multi-epitope vaccine is promising against visceral leishmaniasis. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/ Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas e Genômica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas e Genômica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas e Genômica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brazil. | en_US |
| Subject | BALB/c | en_US |
| Subject | Chimera | en_US |
| Subject | Immune response | en_US |
| Subject | Leishmania infantum | en_US |
| Subject | Multi-epitope vaccine | en_US |
| Subject | Visceral leishmaniasis | en_US |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
