Author | Gomes, Natália Lins da Silva | |
Author | Ruivo, Leonardo Alexandre de Souza | |
Author | Moreira, Claudia | |
Author | Meuser-Batista, Marcelo | |
Author | Silva, Cristiane França da | |
Author | Batista, Denise da Gama Jaen | |
Author | Fragoso, Stênio | |
Author | Oliveira, Gabriel Melo de | |
Author | Soeiro, Maria de Nazaré Correia | |
Author | Moreira, Otacilio C. | |
Access date | 2022-12-14T16:26:23Z | |
Available date | 2022-12-14T16:26:23Z | |
Document date | 2022 | |
Citation | GOMES, Natália Lins da Silva et al. Overexpression of TcNTPDase-1 Gene Increases Infectivity in Mice Infected with Trypanosoma cruzi. International Journal of Molecular Sciences, v. 23, 14661, p. 1 - 18, Nov. 2022. | en_US |
ISSN | 1422-0067 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55936 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | Trypanosoma cruzi | en_US |
Subject in Portuguese | Doença de Chagas | en_US |
Subject in Portuguese | TcNTPDase | en_US |
Subject in Portuguese | Virulência | en_US |
Subject in Portuguese | ecto-NTPDases | en_US |
Title | Overexpression of TcNTPDase-1 Gene Increases Infectivity in Mice Infected with Trypanosoma cruzi | en_US |
Type | Article | |
DOI | 10.3390/ijms232314661 | |
Abstract | Ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes located on the
surface of the T. cruzi plasma membrane, which hydrolyze a wide range of tri-/-diphosphate nucleosides.
In this work, we used previously developed genetically modified strains of Trypanosoma cruzi
(T. cruzi), hemi-knockout (KO +/) and overexpressing (OE) the TcNTPDase-1 gene to evaluate the
parasite infectivity profile in a mouse model of acute infection (n = 6 mice per group). Our results
showed significantly higher parasitemia and mortality, and lower weight in animals infected with
parasites OE TcNTPDase-1, as compared to the infection with the wild type (WT) parasites. On
the other hand, animals infected with (KO +/) parasites showed no mortality during the 30-day
trial and mouse weight was more similar to the non-infected (NI) animals. In addition, they had
low parasitemia (45.7 times lower) when compared with parasites overexpressing TcNTPDase-1
from the hemi-knockout (OE KO +/) group. The hearts of animals infected with the OE KO +/
and OE parasites showed significantly larger regions of cardiac inflammation than those infected
with the WT parasites (p < 0.001). Only animals infected with KO +/ did not show individual
electrocardiographic changes during the period of experimentation. Together, our results expand the
knowledge on the role of NTPDases in T. cruzi infectivity, reenforcing the potential of this enzyme as
a chemotherapy target to treat Chagas disease (CD). | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de PCR em Tempo Real RPT09A, Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de PCR em Tempo Real RPT09A, Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanossomatídeos-ICC. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. EPSJV. Laboratório de Educação Profissional em Técnicas Laboratoriais em Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanossomatídeos-ICC. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de PCR em Tempo Real RPT09A, Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Trypanosoma cruzi | en_US |
Subject | Chagas disease | en_US |
Subject | ecto-NTPDases | en_US |
Subject | TcNTPDase | en_US |
Subject | Virulence | en_US |
xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |