Author | Alladagbin, Dona Jeanne | |
Author | Silva, Carlos Gustavo Regis da | |
Author | Silva, Luciano Kalabric | |
Author | Santos, Washington L. C. dos | |
Author | Oliveira, Geraldo Gileno de Sá | |
Access date | 2022-12-15T16:36:34Z | |
Available date | 2022-12-15T16:36:34Z | |
Document date | 2022 | |
Citation | ALLADAGBIN, Dona Jeanne et al. APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease. BMC Nephrology, v. 23, n. 329, p. 1-7, 2022. | en_US |
ISSN | 1471-2369 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55973 | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). | en_US |
Language | eng | en_US |
Publisher | BMC | en_US |
Rights | open access | en_US |
Subject in Portuguese | Buccal mucosa cells | en_US |
Subject in Portuguese | Sangue | en_US |
Subject in Portuguese | PCR | en_US |
Subject in Portuguese | sequenciamento de DNA | en_US |
Subject in Portuguese | Apolipoproteína L | en_US |
Subject in Portuguese | Haplótipos | en_US |
Title | APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease | en_US |
Type | Article | en_US |
DOI | 10.1186/s12882-022-02954-w | |
Abstract | Two alleles (G1 and G2) of the apolipoprotein 1 gene (APOL1) predispose people of African descent to developing or accelerating the course of certain types of kidney disease. Population studies to determine the frequency of the G1 and G2 alleles are important to inform resource allocation by public health authorities. Traditionally, APOL1 genotyping is carried out in blood samples. However, sample collection, transport, and storage is cumbersome. Here we compared APOL1 genotyping in blood and buccal mucosa cell samples obtained from 23 individuals. Alleles G0 (wild), G1, and G2, as well as genotypes G0/G0, G0/G1, G1/G1, G0/G2, G1/G2, and G2/G2 were detected using both blood and buccal mucosa cells with 100% coincidence. Our data indicate that buccal mucosa cell samples may represent a suitable alternative to blood samples for APOL1 genotyping in the field. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Molecular e Estrutural. Salvador, BA, Brasil. | en_US |
Subject | Buccal mucosa cells | en_US |
Subject | Blood | en_US |
Subject | PCR | en_US |
Subject | DNA sequencing | en_US |
Subject | Apolipoprotein L | en_US |
Subject | Haplotypes | en_US |
DeCS | Sangue | en_US |
DeCS | Reação em Cadeia da Polimerase | en_US |
DeCS | Sequenciamento de Nucleotídeos em Larga Escala | en_US |
DeCS | Apolipoproteína L1 | en_US |
DeCS | Haplótipos | en_US |