| Author | Mesquita, Silvia Gonçalves | |
| Author | Caldeira, Roberta Lima | |
| Author | Favre, Tereza Cristina | |
| Author | Massara, Cristiano Lara | |
| Author | Beck, Lílian Christina Nóbrega Holsbach | |
| Author | Simões, Taynãna César | |
| Author | Carvalho, Gardênia Braz Figueiredo de | |
| Author | Neves, Flória Gabriela dos Santos | |
| Author | Oliveira, Gabriela de | |
| Author | Lacerda, Larisse de Souza Barbosa | |
| Author | Almeida, Matheus Alves de | |
| Author | Carvalho, Omar dos Santos | |
| Author | Mourão, Marina Moraes | |
| Author | Oliveira, Edward | |
| Author | Pereira, Rosane A. Silva | |
| Author | Fonseca, Cristina Toscano | |
| Access date | 2022-12-20T20:17:37Z | |
| Available date | 2022-12-20T20:17:37Z | |
| Document date | 2022 | |
| Citation | MESQUITA, Silvia Gonçalves et al. Assessment of the accuracy of 11 different diagnostic tests for the detection of Schistosomiasis mansoni in individuals from a Brazilian area of low endemicity using latent class analysis. Frontiers in Microbiology, p. 1-20, Dec. 2022. | en_US |
| ISSN | 1664-302X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56098 | |
| Description | This Article is part of the Research Topic
Conference Resarch Topic: 16th International Symposium on Schistosomiasis | en_US |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | |
| Subject in Portuguese | Esquistossomose | en_US |
| Subject in Portuguese | Schistosoma mansoni | en_US |
| Subject in Portuguese | Testes de diagnóstico | en_US |
| Subject in Portuguese | Diagnóstico | en_US |
| Subject in Portuguese | Análise de classe latente | en_US |
| Subject in Portuguese | Pesquisa Clinica | en_US |
| Subject in Portuguese | Sensibilidade | en_US |
| Subject in Portuguese | Especificidade | en_US |
| Title | Assessment of the accuracy of 11 different diagnostic tests for the detection of Schistosomiasis mansoni in individuals from a Brazilian area of low endemicity using latent class analysis | en_US |
| Type | Article | |
| DOI | 10.3389/fmicb.2022.1048457 | |
| Abstract | Background: Schistosomiasis is a parasitic disease associated with poverty. It is estimated that 7.1 million people are infected with Schistosoma mansoni in Latin America, with 95% of them living in Brazil. Accurate diagnosis and timely treatment are important measures to control and eliminate schistosomiasis, but diagnostic improvements are needed to detect infections, especially in areas of low endemicity.
Methodology: This research aimed to evaluate the performance of 11 diagnostic tests using latent class analysis (LCA). A cross-sectional survey was undertaken in a low endemicity area of the municipality of Malacacheta, Minas Gerais, Brazil. Feces, urine, and blood samples were collected from 400 residents older than 6 years of age, who had not been treated with praziquantel in the 12 months previous to the collection of their samples. The collected samples were examined using parasitological (Helm Test® kit Kato-Katz), nucleic acid amplification tests -NAATs (PCR, qPCR and LAMP on urine; PCR-ELISA, qPCR and LAMP on stool), and immunological (POC-CCA, the commercial anti-Schistosoma mansoni IgG ELISA kit from Euroimmun, and two in-house ELISA assays using either the recombinant antigen PPE or the synthetic peptide Smp150390.1) tests. Results: The positivity rate of the 11 tests evaluated ranged from 5% (qPCR on urine) to 40.8% (commercial ELISA kit). The estimated prevalence of schistosomiasis was 12% (95% CI: 9–15%) according to the LCA. Among all tests assessed, the commercial ELISA kit had the highest estimated sensitivity (100%), while the Kato-Katz had the highest estimated specificity (99%). Based on the accuracy measures observed, we proposed three 2-step diagnostic approaches for the active search of infected people in endemic settings. The approaches proposed consist of combinations of commercial ELISA kit and NAATs tests performed on stool. All the approaches had higher sensitivity and specificity than the mean values observed for the 11 tests (70.4 and 89.5%, respectively).
Conclusion: We showed that it is possible to achieve high specificity and sensitivity rates with lower costs by combining serological and NAATs tests, which would assist in the decision-making process for appropriate allocation of public funding aiming to achieve the WHO target of eliminating schistosomiasis as a public health problem by 2030. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Educação em Ambiente e Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Educação em Ambiente e Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René-Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional de Parasitos. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional de Parasitos. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Subject | Schistosomiasis | en_US |
| Subject | Schistosoma mansoni | en_US |
| Subject | Diagnostic tests | en_US |
| Subject | Diagnosis | en_US |
| Subject | Latent class analysis | en_US |
| Subject | Sensitivity | en_US |
| Subject | Specificity | en_US |
| Subject | Clinical research | en_US |
