Author | Villar, Livia Melo | |
Author | Lima, Marjorie Parra de | |
Author | Cruz, Helena Medina | |
Author | Paula, Vanessa Salete de | |
Author | Scalioni, Leticia de Paula | |
Author | Flores, Geane Lopes | |
Author | Costa, Filipe Anibal Carvalho | |
Author | Parente, Cynara Carvalho | |
Author | Coelho, Maria Rosangela Cunha Duarte | |
Author | Albuquerque, Ana Cecilia Cavalcanti de | |
Author | Milagres, Flavio Augusto Pádua | |
Author | Cruz, Marcelo Santos | |
Author | Andrade, Tarcisio Matos | |
Author | Castro, Ana Rita Coimbra Motta | |
Author | Mota, Jurema Corrêa da | |
Author | Lewis-Ximenez, Lia Laura | |
Author | Bastos, Francisco Inácio Pinkusfeld Monteiro | |
Access date | 2022-12-21T17:31:13Z | |
Available date | 2022-12-21T17:31:13Z | |
Document date | 2022 | |
Citation | VILLAR, Livia Melo et al. Feasibility of dried blood spot for hepatitis C diagnosis in vulnerable subjects and people living in remote areas from Brazil. BMC Infectious Diseases, v. 22, 804, p. 1 - 9, 2022. | en_US |
ISSN | 1471-2334 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56135 | |
Language | eng | en_US |
Publisher | BMC | en_US |
Rights | open access | |
Subject in Portuguese | Hepatite C | en_US |
Subject in Portuguese | Mancha de sangue seco | en_US |
Subject in Portuguese | Diagnóstico | en_US |
Subject in Portuguese | Populações vulneráveis | en_US |
Title | Feasibility of dried blood spot for hepatitis C diagnosis in vulnerable subjects and people living in remote areas from Brazil | en_US |
Type | Article | |
DOI | 10.1186/s12879-022-07717-4 | |
Abstract | Background: Agile, accessible and cheap diagnosis of hepatitis C virus (HCV) infection is essential to achieve the
elimination of this infection, worldwide, as mandated by the World Health Organzation as part of its strategy for 2030.
Dried blood spots (DBS) can be an attractive alternative for sample collection among people living in remote areas
and vulnerable populations due to the less invasive collection, its biosafety, and storage & transportation of samples
at room temperature.
Design: This study aims to estimate the usefulness of dried blood spot samples for the diagnosis and the assessment
of HCV infection rates in three different settings in Brazil. Cross-sectional analysis of a sample collection from different
populations, aiming to assess the performance of the testing algorithms and respective procedures among different
populations with diverse background infection rates.
Methods: We reported the evaluation of DBS as alternative samples for detecting anti-HCV in different groups in
real life conditions: (I) Vulnerable subjects living in remote areas of Southeast, North and Northeast Brazil (n = 1464);
(II) Beauticians (n = 288); (III) People who use non-injectable drugs (n = 201); (IV) patients referred to outpatient care
(n = 275).
Results: General assay accuracy was 99%, with a weighted kappa value of 0.9, showing an excellent performance.
Sensitivities ranged from 87.5% to 100.0% between groups and specificities were above 99.2%. A total of 194 individuals
had HCV RNA in serum and concordance of anti-HCV detection in DBS was 98.4%.
Conclusions: DBS samples could be used for anti-HCV detection in different populations recruited in real life conditions
and ambulatory settings, with a high overall sensitivity and specificity. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Universidade Estácio de Sá. Resende, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Ceará. Faculdade de Medicina. Sobral, CE, Brasil. | en_US |
Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Setor de Virologia. Recife, PE, Brasil. | en_US |
Affilliation | Centro Universitário Tabosa de Almeida. Caruaru, PE, Brasil. | en_US |
Affilliation | Universidade Federal de Tocantins. Faculdade de Medicina. Palmas, TO, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Psiquiatria. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal da Bahia. Departamento de Saúde Comunitária e Familiar. Salvador, BA, Brasil. | en_US |
Affilliation | Universidade Federal de Mato Grosso do Sul. Campo Grande, MS, Brasil / Fundação Oswaldo Cruz-MS. Campo Grande, MS, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnologia para a Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnologia para a Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Hepatitis C | en_US |
Subject | Dried blood spot | en_US |
Subject | Diagnosis | en_US |
Subject | Vulnerable populations | en_US |