| Author | Costa, Emmanoel V. | |
| Author | Souza, César A. S. de | |
| Author | Galvão, Alexandre F. C. | |
| Author | Silva, Valdenizia R. | |
| Author | Santos, Luciano de S. | |
| Author | Dias, Rosane B. | |
| Author | Rocha, Clarissa A. Gurgel | |
| Author | Soares, Milena B. P. | |
| Author | Silva, Felipe M. A. da | |
| Author | Koolen, Hector H. F. | |
| Author | Bezerra, Daniel P. | |
| Access date | 2022-12-21T18:32:18Z | |
| Available date | 2022-12-21T18:32:18Z | |
| Document date | 2022 | |
| Citation | Costa, Emmanoel V. et al. Duguetia pycnastera sandwith (Annonaceae) leaf essential oil inhibits HepG2 cell growth in vitro and in vivo. Molecules, v. 27, p. 1-14, 2022. | en_US |
| ISSN | 1420-3049 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56141 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Duguetia pycnastera | en_US |
| Subject in Portuguese | Óleo essencial | en_US |
| Subject in Portuguese | Células HepG2 | en_US |
| Subject in Portuguese | Citotóxico | en_US |
| Subject in Portuguese | antitumoral | en_US |
| Title | Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo | en_US |
| Type | Article | en_US |
| DOI | 10.3390/molecules 27175664 | |
| Abstract | Abstract: Duguetia pycnastera Sandwith (Annonaceae) is a tropical tree that can be found in the Guyanas, Bolivia, Venezuela, and Brazil. In Brazil, it is popularly known as “ata”, “envira”, “envirapreta”, and “envira-surucucu”. In the present work, we investigated the in vitro and in vivo HepG2 cell growth inhibition capacity of D. pycnastera leaf essential oil (EO). The chemical composition of the EO was determined by GC–MS and GC–FID analyses. The alamar blue assay was used to examine the in vitro cytotoxicity of EO in cancer cell lines and non-cancerous cells. In EO-treated HepG2 cells, DNA fragmentation was measured by flow cytometry. The in vivo antitumor activity of the EO was assessed in C.B-17 SCID mice xenografted with HepG2 cells treated with the EO at a dosage of 40 mg/kg. Chemical composition analysis displayed the sesquiterpenes -gurjunene (26.83%), bicyclogermacrene (24.90%), germacrene D (15.35%), and spathulenol (12.97%) as the main EO constituents. The EO exhibited cytotoxicity, with IC50 values ranging from 3.28 to 39.39 g/mL in the cancer cell lines SCC4 and CAL27, respectively. The cytotoxic activity of the EO in non-cancerous
cells revealed IC50 values of 16.57, 21.28, and >50 g/mL for MRC-5, PBMC, and BJ cells, respectively. An increase of the fragmented DNA content was observed in EO-treated HepG2 cells. In vivo, EO displayed tumor mass inhibition activity by 47.76%. These findings imply that D. pycnastera leaf EO may have anti-liver cancer properties. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil. | en_US |
| Affilliation | Instituto SENAI de Inovação em Sistemas Avançados em Saúde. Centro Universitário SENAI/CIMATEC. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Estadual do Amazonas. Grupo de Pesquisa em Metabolômica e Espectrometria de Massas. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Subject | Duguetia pycnastera | en_US |
| Subject | Essential oil | en_US |
| Subject | HepG2 cells | en_US |
| Subject | Cytotoxic | en_US |
| Subject | Antitumor | en_US |
| DeCS | Óleos Voláteis | en_US |
| DeCS | Células Hep G2 | en_US |
| DeCS | Citotoxinas | en_US |
