| Author | Rangel, Karyne | |
| Author | Cabral, Fellipe O. | |
| Author | Lechuga, Guilherme C. | |
| Author | Villas-Bôas, Maria H. S. | |
| Author | Midlej, Victor | |
| Author | De-Simone, Salvatore G. | |
| Access date | 2022-12-30T12:30:38Z | |
| Available date | 2022-12-30T12:30:38Z | |
| Document date | 2022 | |
| Citation | RANGEL, Karine et al. Effectiveness Evaluation of a UV-C-Photoinactivator against Selected ESKAPE-E Pathogens. Int. J. Environ. Res. Public Health, v. 19, 16559, p. 1- 19, Dec. 2022. | en_US |
| ISSN | 1661-7827 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56226 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | UV-C | en_US |
| Subject in Portuguese | Descontaminador de sola de sapato | en_US |
| Subject in Portuguese | Patógenos ESKAPE-E | en_US |
| Subject in Portuguese | Resistência a múltiplas drogas | en_US |
| Subject in Portuguese | Desinfecção | en_US |
| Subject in Portuguese | Viabilidade celular | en_US |
| Subject in Portuguese | SEM | en_US |
| Title | Effectiveness Evaluation of a UV-C-Photoinactivator against Selected ESKAPE-E Pathogens | en_US |
| Type | Article | |
| DOI | 10.3390/ ijerph192416559 | |
| Abstract | Healthcare-associated infections (HAI) worldwide includes infections by ESKAPE-E
pathogens. Environmental surfaces and fomites are important components in HAI transmission
dynamics, and shoe soles are vectors of HAI. Ultraviolet (UV) disinfection is an effective method
to inactivate pathogenic microorganisms. In this study, we investigated whether the SANITECH
UV-C shoe sole decontaminator equipment that provides germicidal UV-C radiation could effectively
reduce this risk of different pathogens. Six standard strains and four clinical MDR strains
in liquid and solid medium were exposed to a UV-C System at specific concentrations at other
times. Bacterial inactivation (growth and cultivability) was investigated using colony counts and
resazurin as metabolic indicators. SEM was performed to assess the membrane damage. Statistically
significant reduction in cell viability for all ATCCs strains occurred after 10 s of exposure to the UV-C
system, except for S. enterica, which only occurred at 20 s. The cell viability of P. aeruginosa (90.9%),
E. faecalis and A. baumannii (85.3%), S. enterica (82.9%), E. coli (79.2%) and S. aureus (71.9%) was
reduced considerably at 20 s. In colony count, after 12 s of UV-C exposure, all ATCC strains showed
a 100% reduction in CFU counts, except for A. baumannii, which reduced by 97.7%. A substantial
reduction of colonies above 3 log10 was observed at 12 and 20 s in all bacterial strains tested, except for
A. baumannii ATCC 19606 (12 s). The exposure of ATCCs bacterial strains to the UV-C system for only
2 s was able to reduce 100% in the colony forming units (CFU) count in all ATCCs strains, S. aureus,
P. aeruginosa, E. coli, A. baumannii, E. faecalis, except the S. enterica strain which had a statistically
significant reduction of 99.7%. In ATCC strains, there was a substantial decrease in colonies after 4 s
(sec) of exposure to the UV-C system, with a reduction ranging from 3.78–4.15 log10 CFU/mL. This
reduction was observed in MDR/ESKAPE-E strains within 10 s, showing that UV-C could eliminate
above 3.84 log10 CFU/mL. SEM showed a reduction of pili-like appendages after UV-C treatment in
all strains except for E. coli (ATCC 25922). The Sanitech UV-C shoe sole decontaminator equipment
from Astech Serv. and Fabrication Ltd. (Petrópolis, Brazil), effectively killed in vitro a series of ATCCs
and MDR/ESKAPE-E bacteria of sanitary interest, commonly found in the hospital environment. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Populacionais Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Populacionais Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departametno de Microbiologia. Rio de Janeiro, RJ, Brasil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Populacionais Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Programa de Pós Graduação em Ciência e Biotecnologia. Niterói, RJ, Brasil. | en_US |
| Subject | UV-C | en_US |
| Subject | Shoe sole decontaminator | en_US |
| Subject | ESKAPE-E pathogens | en_US |
| Subject | Multidrug resistance | en_US |
| Subject | Disinfection | en_US |
| Subject | Cell viability | en_US |
| Subject | SEM | en_US |
