| Author | Alves-Sobrinho, Ednéia Venâncio | |
| Author | Pinheiro, Lucélia de Jesus | |
| Author | Paranaíba, Larissa Ferreira | |
| Author | Fontes, Igor Campos | |
| Author | Parreiras, Patrícia Martins | |
| Author | Gontijo, Nelder Figueiredo | |
| Author | Tafuri, Wagner Luiz | |
| Author | Laurenti, Márcia Dalastra | |
| Author | Soares, Rodrigo Pedro Pinto | |
| Access date | 2023-01-05T19:03:12Z | |
| Available date | 2023-01-05T19:03:12Z | |
| Document date | 2022 | |
| Citation | ALVES-SOBRINHO, Ednéia Venâncio et al. Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus. Mem Inst Oswaldo Cruz, Rio de Janeiro, v. 117, e220065, 2022. doi: 10.1590/0074-02760220065. | en_US |
| ISSN | 1678-8060 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56336 | |
| Language | eng | en_US |
| Publisher | Instituto Oswaldo Cruz | en_US |
| Rights | open access | en_US |
| Title | Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus | en_US |
| Type | Article | en_US |
| Abstract | BACKGROUND: Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle.
OBJECTIVE: Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host.
METHODS: Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene.
FINDINGS: Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations.
MAIN CONCLUSION: Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil/ Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Moléstias Infecciosas. São Paulo, SP, Brasil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil | en_US |
| Subject | Leishmania enriettii | en_US |
| Subject | Cavia porcellus | en_US |
| Subject | subgenus Mundinia | en_US |
| Subject | visceralisation | en_US |
| Subject | histopathology | en_US |
| Subject | host-parasite interaction | en_US |
