Author | Ferreira, Luiz Cláudio | |
Author | Quintella, Leonardo Pereira | |
Author | Schubach, Armando de Oliveira | |
Author | Miranda, Luciana de Freitas Campos | |
Author | Madeira, Maria de Fátima | |
Author | Pimentel, Maria Inês Fernandes | |
Author | Ferreira e Vasconcellos, Érica de Camargo | |
Author | Lyra, Marcelo Rosandiski | |
Author | Oliveira, Raquel de Vasconcellos Carvalhaes de | |
Author | Menezes, Rodrigo Caldas | |
Access date | 2023-01-09T12:39:24Z | |
Available date | 2023-01-09T12:39:24Z | |
Document date | 2022 | |
Citation | FERREIRA, Luiz Cláudio et al. Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples. Tropical medicine and infectious disease, v. 7, n. 11, p. 1-13, 2022. | en_US |
ISSN | 2414-6366 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56390 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples | en_US |
Type | Article | en_US |
DOI | 10.3390/tropicalmed7110344 | |
Abstract | New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan Leishmania. Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of Leishmania spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by L. (V.) braziliensis. These specimens were analyzed by CISH, using a generic probe for Leishmania, IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by L. (V.) braziliensis. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Técnico Educacional Souza Marques. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos . Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Leishmania braziliensis | en_US |
Subject | Diagnosis | en_US |
Subject | Immunoperoxidase | en_US |
Subject | In situ hybridization | en_US |
Subject | Tegumentary leishmaniasis | en_US |