| Author | Cipitelli, Marcio da Costa | |
| Author | Paiva, Iury Amancio | |
| Author | Badolato-Corrêa, Jéssica | |
| Author | Marinho, Cíntia Ferreira | |
| Author | Solórzano, Victor Edgar Fiestas | |
| Author | Faria, Nieli Rodrigues da Costa | |
| Author | Azeredo, Elzinandes Leal de | |
| Author | Souza, Luiz José de | |
| Author | Cunha, Rivaldo Venâncio da | |
| Author | PInto, Luzia Maria de Oliveira | |
| Access date | 2023-01-10T17:52:16Z | |
| Available date | 2023-01-10T17:52:16Z | |
| Document date | 2022 | |
| Citation | CIPITELLI, Marcio da Costa et al. Subsets of Cytokines and Chemokines from DENV-4-Infected Patients Could Regulate the Endothelial Integrity of Cultured Microvascular Endothelial Cells. Pathogens, v. 11, 509, p. 1 - 18, Apr. 2022. | en_US |
| ISSN | 2076-0817 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56424 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Dengue | en_US |
| Subject in Portuguese | Citocinas | en_US |
| Subject in Portuguese | Quimiocinas | en_US |
| Subject in Portuguese | Células endoteliais | en_US |
| Subject in Portuguese | Permeabilidade | en_US |
| Title | Subsets of Cytokines and Chemokines from DENV-4-Infected Patients Could Regulate the Endothelial Integrity of Cultured Microvascular Endothelial Cells | en_US |
| Type | Article | |
| DOI | 10.3390/ pathogens11050509 | |
| Abstract | Abstract: Introduction: It is a consensus that inflammatory mediators produced by immune cells
contribute to changes in endothelial permeability in dengue. We propose to relate inflammatory
mediators seen in dengue patients with the in vitro alteration of endothelial cells (ECs) cultured
with serum from these patients. Methods: Patients with mild (DF) to moderate and severe dengue
(DFWS/Sev) were selected. ELISA quantified inflammatory mediators. Expression of adhesion
molecules and CD147 were evaluated in the ECs cultured with the patient’s serum by flow cytometry.
We assessed endothelial permeability by measuring transendothelial electrical resistance in
cocultures of ECs with patient serum. Results: Dengue infection led to an increase in inflammatory
mediators—the IL-10 distinguished DF from DFWS/Sev. There were no changes in CD31, CD54,
and CD106 but decreased CD147 expression in ECs. DFWS/Sev sera induced a greater difference
in endothelial permeability than DF sera. Correlation statistical test indicated that low IL-10 and
IFN-
and high CCL5 maintain the integrity of ECs in DF patients. In contrast, increased TNF, IFN-
,
CXCL8, and CCL2 maintain EC integrity in DFWS/Sev patients. Conclusions: Our preliminary
data suggest that a subset of inflammatory mediators may be related to the maintenance or loss of
endothelial integrity, reflecting the clinical prognosis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Centro de Referência de Doenças Imuno-Infecciosas. Campos dos Goytacazes, RJ, Brasil / Faculdade de Medicina de Campos. Departamento de Clínica Médica. Campos dos Goytacazes, RJ, Brasil, | en_US |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Campo Grande, MS, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Dengue | en_US |
| Subject | Cytokines | en_US |
| Subject | Chemokines | en_US |
| Subject | Endothelial cells | en_US |
| Subject | Permeability | en_US |
