Author | Costa, Vanessa Duarte da | |
Author | Santos, Alanna Calheiros | |
Author | Silva, Lucas Lima da | |
Author | Wiggers, Wilian Jean | |
Author | Ivantes, Claudia Alexandra Pontes | |
Author | Lima, Danielle Malta | |
Author | Colares, Jeová Keny Baima | |
Author | Dallacqua, Deusilene Souza Vieira | |
Author | Castro, Ana Rita Coimbra Motta | |
Author | Rivera Dávila, Alberto Martín | |
Author | Teles, Sheila Araujo | |
Author | Carneiro, Megmar Aparecida dos Santos | |
Author | Caetano, Karlla Antonieta Amorim | |
Author | Anunciação, Fernando Antonio Costa | |
Author | Paula, Vanessa Salete de | |
Author | Villar, Livia Melo | |
Access date | 2023-01-19T11:18:32Z | |
Available date | 2023-01-19T11:18:32Z | |
Document date | 2023 | |
Citation | COSTA, Vanessa Duarte da et al. RT-LAMP Multicenter Study for SARS-CoV-2 Genome Molecular Detection in Brazilian Swab and Saliva Samples. Diagnostics, v. 13, 210, p. 1-13, Jan. 2023. | en_US |
ISSN | 2075-4418 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56508 | |
Description | Behalf of The Brazilian COVID-19 Research Group: Aline Pedroso Lorenz (Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil); Crhistinne Cavalheiro Maymone Gonçalves (Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil); Wellington Santos Fava (Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil); James Venturini (Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil); Luiz Demarchi (Central Public Health Laboratory of the State of Mato Grosso do Sul (LACEN-MS), Campo Grande, Mato Grosso do Sul, Brazil). | |
Sponsorship | This research was funded by the Oswaldo Cruz Foundation (FIOCRUZ) via INOVA FIOCRUZ VPPCB-005-FIO-20-2-52 program grant number 484015522386498, the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ) grant numbers E26/204.196, E-26/204.197/2021, and E-26/200.821/202, the Higher Education Personnel Improvement Coordination (CAPES) grant numbers 88887.661289/2022-00 and 88887.661288/2022-00, and the National Council for Scientific and Technological Development (CNPq) grant number 315967/2021-8. | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | LAMP | en_US |
Subject in Portuguese | SARS-CoV-2 | en_US |
Subject in Portuguese | COVID-19 | en_US |
Subject in Portuguese | Diagnóstico | en_US |
Title | RT-LAMP Multicenter Study for SARS-CoV-2 Genome Molecular Detection in Brazilian Swab and Saliva Samples | en_US |
Type | Article | |
DOI | 10.3390/diagnostics13020210 | |
Abstract | Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid method that can replace RT-qPCR. A simple molecular assay for SARS-CoV-2 RNA detection in gold-standard diagnosis through swabs and alternative specimens such as saliva could be helpful in promoting genomic surveillance. A multicenter study was conducted to evaluate the RT-LAMP assay method as an alternative for the molecular detection of SARS-CoV-2 lineages in swab and saliva samples. A total of 350 swabs from individuals with (n = 276) or without (n = 74) COVID-19 tested by RT-qPCR were collected. Paired saliva was also collected from 90 individuals who had SARS-CoV-2 RNA that was detectable (n = 30) or undetectable (n = 60) via RT-qPCR. For the RT-LAMP methodology, six primers were used for ORF1 gene amplification. As for SARS-CoV-2 genotyping, 39 swabs had the whole genome sequenced by MinION. The sensitivity of RT-LAMP to the swab was 90.2%. For the swab samples with Ct 30, the sensitivity improved by 96%. Considering saliva with Ct 30 in RT-qPCR testing, the RT-LAMP sensitivity was 100%. The RT-LAMP specificity was 100% for both the swab and saliva samples. This RT-LAMP assay was capable of detecting all the SARS-CoV-2 lineages circulating in the Brazilian swab samples. The RT-LAMP method has significant potential for use in clinical routines since it was capable of detecting SARS-CoV-2 RNA in swab and saliva samples. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Brasileiro de Referência em Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Brasileiro de Referência em Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Brasileiro de Referência em Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Hospital Nossa Senhora das Graças. Serviço de Gastroenterologia, Hepatologia e Transplante Hepático. Curitiba, PR, Brasil. | en_US |
Affilliation | Hospital Nossa Senhora das Graças. Serviço de Gastroenterologia, Hepatologia e Transplante Hepático. Curitiba, PR, Brasil. | en_US |
Affilliation | Universidade de Fortaleza. Programa de Pós-Graduação em Ciências Médicas. Fortaleza, CE, Brasil. | en_US |
Affilliation | Universidade de Fortaleza. Programa de Pós-Graduação em Ciências Médicas. Fortaleza, CE, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal de Mato Grosso do Sul. Campo Grande, MS, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Laboratório de Biologia Computacional e de Sistemas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal de Goiás. Faculdade Federal. Goiânia, GO, Brasil. | en_US |
Affilliation | Universidade Federal de Goiás. Faculdade Federal. Goiânia, GO, Brasil. | en_US |
Affilliation | Universidade Federal de Goiás. Faculdade Federal. Goiânia, GO, Brasil. | en_US |
Affilliation | Hospital Getúlio Vargas. Teresina, PI, Brasil / Instituto Natan Portela de Doenças Tropicais. Teresina, PI, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Brasileiro de Referência em Hepatites Virais. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | LAMP | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | COVID-19 | en_US |
Subject | Diagnosis | en_US |