| Author | Schriefer, Albert | |
| Author | Maltez, J. R | |
| Author | Silva, N | |
| Author | Stoeckle, M.Y | |
| Author | Barral Netto, Manoel | |
| Author | Riley, Lee Woodland | |
| Access date | 2012-10-05T18:51:53Z | |
| Available date | 2012-10-05T18:51:53Z | |
| Document date | 1999 | |
| Citation | SCHRIEFER, A. et al. Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. Vaccine, v. 17, n. 7-8, p. 770-8, feb. 1999. | pt_BR |
| ISSN | 0264-410X | |
| URI | https://www.arca.fiocruz.br/handle/icict/5652 | |
| Language | eng | pt_BR |
| Rights | open access | |
| Title | Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. | pt_BR |
| Type | Article | pt_BR |
| Abstract | Enteropathogenic Escherichia coli (EPEC) is a major cause of childhood diarrhea in developing countries and is a leading cause of severe diarrheal illness among Brazilian infants. As one approach to constructing a vaccine candidate against diarrhea caused by EPEC, we evaluated whether the pilin subunit (BfpA) of the bundle-forming pilus (BFP) could be expressed by a live Salmonella vaccine strain. Several copies of the coding region of BfpA (bfpA) were amplified by PCR from a preparation of the EAF plasmid of EPEC strain B171 and cloned into plasmid vectors. An intact copy of bfpA was subcloned into the heat inducible prokaryotic expression vector pCYTEXP1, and the resulting pBfpA was used to transform the aroA S. typhimurium strain SL3261, generating SL3261(pBfpA). The recombinant vaccine strain was able to express, but not to process, rBfpA as evidenced by a prominent 21 kDa protein that crossreacted with anti-BFP antiserum found only in extracts of heat-treated SL3261(pBfpA), but not in strains of untreated SL3261(pBfpA) or SL3261 not carrying the plasmid. Furthermore, rBfpA accumulation was not toxic to the Salmonella host, as evidenced by similar plating efficiencies between induced and uninduced strains of SL3261(pBfpA). Finally, SL3261(pBfpA) orally administered to BALB/c mice was capable of eliciting a sustained and vigorous humoral immune response to BfpA, achievable even with a single oral dose of approximately 10(9) organisms. Therefore, this pilin product may serve as a potential immunogen as part of a live combined vaccine strategy to prevent two of the major public health problems in Brazil--salmonellosis and EPEC childhood diahrrea. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Cornell University Medical College. Division of International Medicine and Infectious Diseases. New York, New York | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Cornell University Medical College. Division of International Medicine and Infectious Diseases. New York, New York | pt_BR |
| Subject | EPEC | pt_BR |
| Subject | BFP | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | Oral | pt_BR |
| DeCS | Proteínas da Membrana Bacteriana Externa/genética | pt_BR |
| DeCS | Vacinas Bacterianas/imunologia | pt_BR |
| DeCS | Proteínas de Escherichia coli | pt_BR |
| DeCS | Escherichia coli/genética | pt_BR |
| DeCS | Escherichia coli/imunologia | pt_BR |
| DeCS | Fímbrias Bacterianas/imunologia | pt_BR |
| DeCS | Proteínas de Membrana/genética | pt_BR |
| DeCS | Vacinas contra Salmonella | pt_BR |
| DeCS | Salmonella typhimurium/imunologia | pt_BR |
| DeCS | Vacinas Tíficas-Paratíficas | pt_BR |
| DeCS | Administração Oral | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Anticorpos Antibacterianos/biossíntese | pt_BR |
| DeCS | Especificidade de Anticorpos | pt_BR |
| DeCS | Proteínas da Membrana Bacteriana | pt_BR |
| DeCS | Externa/biossíntese | pt_BR |
| DeCS | Vacinas Bacterianas/genética | pt_BR |
| DeCS | Clonagem Molecular | pt_BR |
| DeCS | Escherichia coli/patogenicidade | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Proteínas de Fímbrias | pt_BR |
| DeCS | Fímbrias Bacterianas/genética | pt_BR |
| DeCS | Regulação Bacteriana da Expressão | pt_BR |
| DeCS | Gênica/imunologia | pt_BR |
| DeCS | Vetores Genéticos/imunologia | pt_BR |
| DeCS | Proteínas de Membrana/imunologia | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
| DeCS | Salmonella typhimurium/genética | pt_BR |
| DeCS | Transformação Bacteriana | pt_BR |
| DeCS | Vacinas Atenuadas/genética | pt_BR |
| DeCS | Vacinas Atenuadas/imunologia | pt_BR |
| DeCS | Vacinas Sintéticas/imunologia | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
