Author | Reis, Jordana Grazziela Alves Coelho dos | |
Author | Ferreira, Geovane Marques | |
Author | Lourenço, Alice Aparecida | |
Author | Ribeiro, Ágata Lopes | |
Author | Mata, Camila Pacheco da Silveira Martins da | |
Author | Oliveira, Patrícia de Melo | |
Author | Marques, Daisymara Priscila de Almeida | |
Author | Ferreira, Linziane Lopes | |
Author | Clarindo, Felipe Alves | |
Author | Silva, Murillo Ferreira da | |
Author | Póvoas Filho, Heitor Portella | |
Author | Oliveira Junior, Nilson Roberto Ribeiro | |
Author | Sodré, Maisah Meyhr D'Carmo | |
Author | Gadelha, Sandra Rocha | |
Author | Albuquerque, George Rego | |
Author | Maciel, Bianca Mendes | |
Author | Mariano, Ana Paula Melo | |
Author | Silva, Mylene de Melo | |
Author | Fontana, Renato | |
Author | Marin, Lauro Juliano | |
Author | Carlos, Renata Santiago Alberto | |
Author | Lopes, Amanda Teixeira Sampaio | |
Author | Ferreira, Fabrício Barbosa | |
Author | Santos, Uener Ribeiro Dos | |
Author | Santana, Íris Terezinha Santos de | |
Author | Fehlberg, Hllytchaikra Ferraz | |
Author | Rezende, Rachel Passos | |
Author | Dias, João Carlos T | |
Author | Gross, Eduardo | |
Author | Goulart, Gisele Assis Castro | |
Author | Santiago, Marie Gabriele | |
Author | Lemos, Ana Paula Motta Lavigne de | |
Author | Conceição, Aline O da | |
Author | Romano, Carla Cristina | |
Author | Carvalho, Luciana Debortoli de | |
Author | Martins Filho, Olindo Assis | |
Author | Quadros, Claudio Almeida | |
Author | Morris, David L | |
Author | Valle, Sarah J | |
Access date | 2023-01-23T18:16:08Z | |
Available date | 2023-01-23T18:16:08Z | |
Document date | 2022 | |
Citation | REIS, Jordana Grazziela A Coelho Dos et al. Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19. Biomed Pharmacother., v. 148, 112753, 2022. doi: 10.1016/j.biopha.2022.112753. | en_US |
ISSN | 0753-3322 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56561 | |
Language | eng | en_US |
Publisher | Editions Scientifiques Elsevier | en_US |
Rights | open access | en_US |
Title | Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19 | en_US |
Type | Article | en_US |
Abstract | COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2, which continues to be a public health threat. COVID-19 is principally a respiratory disease and is often associated with sputum retention and cytokine storm, for which there are limited therapeutic options. In this regard, we evaluated the use of BromAc®, a combination of Bromelain and Acetylcysteine (NAC). Both drugs present mucolytic effect and have been studied to treat COVID-19. Therefore, we sought to examine the mucolytic and anti-inflammatory effect of BromAc® in tracheal aspirate samples from critically ill COVID-19 patients requiring mechanical ventilation.
Method: Tracheal aspirate samples from COVID-19 patients were collected following next of kin consent and mucolysis, rheometry and cytokine analysis using Luminex kit was performed.
Results: BromAc® displayed a robust mucolytic effect in a dose dependent manner on COVID-19 sputum ex vivo. BromAc® showed anti-inflammatory activity, reducing the action of cytokine storm, chemokines including MIP-1alpha, CXCL8, MIP-1b, MCP-1 and IP-10, and regulatory cytokines IL-5, IL-10, IL-13 IL-1Ra and total reduction for IL-9 compared to NAC alone and control. BromAc® acted on IL-6, demonstrating a reduction in G-CSF and VEGF-D at concentrations of 125 and 250 µg.
Conclusion: These results indicate robust mucolytic and anti-inflammatory effect of BromAc® ex vivo in tracheal aspirates from critically ill COVID-19 patients, indicating its potential to be further assessed as pharmacological treatment for COVID-19. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Risoleta Tolentino Neves Hospital. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Hospital de Ilhéus. Ilhéus, BA, Brazil. | en_US |
Affilliation | Hospital de Ilhéus. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Agricultural and Environmental Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Health Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Agricultural and Environmental Science. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Hospital de Ilhéus. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Hospital de Ilhéus. Ilhéus, BA, Brazil. | en_US |
Affilliation | Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil. | en_US |
Affilliation | Grupo Integrado de Pesquisas em Biomarcadores. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | São Rafael Hospital. Salvador, Bahia, Brazil/Bahia State University. Salvador, Bahia, Brazil. | en_US |
Affilliation | Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia/Department of Surgery. St George Hospital, Sydney.NSW, Australia. | en_US |
Affilliation | Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia. | en_US |
Subject | BromAc | en_US |
Subject | COVID-19 | en_US |
Subject | Mucolytic therapy | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | Tracheal aspirate | en_US |