| Author | Pontes, Gina | |
| Author | Latorre-Estivalis, José Manuel | |
| Author | Gutiérrez, María Laura | |
| Author | Cano, Agustina | |
| Author | Astrada, Martin Berón de | |
| Author | Lorenzo, Marcelo Gustavo | |
| Author | Barrozo, Romina B | |
| Access date | 2023-01-25T19:33:08Z | |
| Available date | 2023-01-25T19:33:08Z | |
| Document date | 2022 | |
| Citation | PONTES, Gina et al. Molecular and functional basis of high-salt avoidance in a blood-sucking insect. iScience, v. 25, n. 7, 104502, 2022. doi: 10.1016/j.isci.2022.104502 | en_US |
| ISSN | 2589-0042 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56623 | |
| Language | eng | en_US |
| Publisher | Cell Press | en_US |
| Rights | restricted access | en_US |
| Title | Molecular and functional basis of high-salt avoidance in a blood-sucking insect | en_US |
| Type | Article | en_US |
| Abstract | Salts are essential nutrients required for many physiological processes, and accordingly, their composition and concentration are tightly regulated. Taste is the ultimate sensory modality involved in resource quality assessment, resulting in acceptance or rejection. Here we found that high salt concentrations elicit feeding avoidance in the blood-sucking bug Rhodnius prolixus and elucidate the molecular and neurophysiological mechanisms involved. We found that high-salt avoidance is mediated by a salt-sensitive antennal gustatory receptor neuron (GRN). Using RNAi, we demonstrate that this process requires two amiloride-sensitive pickpocket channels (PPKs; Rpro PPK014276 and Rpro PPK28) expressed within these cells. We found that antennal GRNs project to the insect primary olfactory center, the antennal lobes, revealing these centers as potential sites for the integration of taste and olfactory host-derived cues. Moreover, the identification of the gustatory basis of high-salt detection in a hematophagous insect suggests novel targets for the prevention of biting and feeding. | en_US |
| Affilliation | Grupo de Neuroetología de Insectos Vectores. Laboratorio Fisiología de Insectos. Instituto de Biodiversidad, Biología Experimental y Aplicada. CONICET – UBA/Departamento Biodiversidad y Biología Experimental. Facultad Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Grupo de Neuroetología de Insectos Vectores. Laboratorio Fisiología de Insectos. Instituto de Biodiversidad, Biología Experimental y Aplicada. CONICET – UBA/Departamento Biodiversidad y Biología Experimental. Facultad Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | en_US |
| Affilliation | Grupo de Neuroetología de Insectos Vectores. Laboratorio Fisiología de Insectos. Instituto de Biodiversidad, Biología Experimental y Aplicada. CONICET – UBA/Departamento Biodiversidad y Biología Experimental. Facultad Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | en_US |
| Affilliation | Laboratorio de Fisiología de la Visión. Instituto de Biociencias, Biotecnología y Biología Traslacional. Departamento de Fisiología, Biología Molecular y Celular. Facultad Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Grupo de Neuroetología de Insectos Vectores. Laboratorio Fisiología de Insectos. Instituto de Biodiversidad, Biología Experimental y Aplicada. CONICET – UBA/Departamento Biodiversidad y Biología Experimental. Facultad Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | en_US |
| Subject | Biological sciences | en_US |
| Subject | Neuroscience | en_US |
| Subject | Sensory neuroscience | en_US |
| Embargo date | 2025-12-31 | |
