Author | Rezende, Izabela Maurício de | |
Author | Oliveira, Gabriela Fernanda Garcia | |
Author | Costa, Thaís Alkifeles | |
Author | Khan, Aslam | |
Author | Pereira, Leonardo Soares | |
Author | Santos, Tayrine Araújo | |
Author | Alves, Pedro Augusto | |
Author | Silva, Carlos Eduardo Calzavara | |
Author | Martins Filho, Olindo Assis | |
Author | Carvalho, Andréa Teixeira de | |
Author | LaBeaud, Angelle Desiree | |
Author | Drumond, Betânia Paiva | |
Access date | 2023-02-01T14:07:57Z | |
Available date | 2023-02-01T14:07:57Z | |
Document date | 2022 | |
Citation | REZENDE, Izabela Maurício de et al. Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease. J Clin Microbiol., v. 60, n. 8, e0025422, 2022. doi: 10.1128/jcm.00254-22. | en_US |
ISSN | 0095-1137 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56807 | |
Language | eng | en_US |
Publisher | American Society for Microbiology | en_US |
Rights | open access | en_US |
Title | Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease | en_US |
Type | Article | en_US |
Abstract | Prior studies have demonstrated prolonged presence of yellow fever virus (YFV) RNA in saliva and urine as an alternative to serum. To investigate the presence of YFV RNA in urine, we used RT-PCR for YFV screening in 60 urine samples collected from a large cohort of naturally infected yellow fever (YF) patients during acute and convalescent phases of YF infection from recent YF outbreaks in Brazil (2017 to 2018). Fifteen urine samples from acute phase infection (up to 15 days post-symptom onset) and four urine samples from convalescent phase infection (up to 69 days post-symptom onset), were YFV PCR-positive. We genotyped YFV detected in seven urine samples (five collected during the acute phase and two collected during the YF convalescent phase). Genotyping indicated the presence of YFV South American I genotype in these samples. To our knowledge, this is the first report of wild-type YFV RNA detection in the urine this far out from symptom onset (up to 69 DPS), including YFV RNA detection during the convalescent phase of YF infection. The detection of YFV RNA in urine is an indicative of YFV infection; however, the results of RT-PCR using urine as sample should be interpreted with care, since a negative result does not exclude the possibility of YFV infection. With a possible prolonged period of detection beyond the viremic phase, the use of urine samples coupled with serological tests, epidemiologic inquiry, and clinical assessment could provide a longer diagnostic window for laboratory YF diagnosis. | en_US |
Affilliation | Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/ Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA | en_US |
Affilliation | Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA. | en_US |
Affilliation | Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Immunology of Viruses Diseases. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Cellular and Molecular Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Integrated Group of Biomarkers Research. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Integrated Group of Biomarkers Research. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA. | en_US |
Affilliation | Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Subject | diagnosis | en_US |
Subject | urine | en_US |
Subject | virus surveillance | en_US |
Subject | yellow fever | en_US |
Subject | yellow fever vírus | en_US |