| Author | Rossi, Izadora Volpato | |
| Author | Nunes, Maria Alice Ferreira | |
| Author | Sabatke, Bruna | |
| Author | Ribas, Hennrique Taborda | |
| Author | Winnischofer, Sheila Maria Brochado | |
| Author | Ramos, Augusto Savio Peixoto | |
| Author | Inal, Jameel Malhador | |
| Author | Ramirez, Marcel Ivan | |
| Access date | 2023-02-03T15:48:47Z | |
| Available date | 2023-02-03T15:48:47Z | |
| Document date | 2022 | |
| Citation | ROSSI, Izadora Volpato et al. An induced population of Trypanosoma cruzi epimastigotes more resistant to complement lysis promotes a phenotype with greater differentiation, invasiveness, and release of extracellular vesicles. Front. Cell. Infect. Microbiol, v. 12, n. 1046681. p. 1–12, 2022. | en_US |
| ISSN | 2235-2988 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/56856 | |
| Language | por | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Infecção por Trypanosoma cruzi | en_US |
| Title | An induced population of Trypanosoma cruzi epimastigotes more resistant to complement lysis promotes a phenotype with greater differentiation, invasiveness, and release of extracellular vesicles | en_US |
| Type | Article | en_US |
| Abstract | Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi, which uses blood-feeding triatomine bugs as a vector to finally infect mammalian hosts. Upon entering the host, the parasite needs to effectively evade the attack of the complement system and quickly invade cells to guarantee an infection. In order to accomplish this, T. cruzi expresses different molecules on its surface and releases extracellular vesicles (EVs). Here, we have selected a population of epimastigotes (a replicative form) from T. cruzi through two rounds of exposure to normal human serum (NHS), to reach 30% survival (2R population). This 2R population was characterized in several aspects and compared to Wild type population. The 2R population had a favored metacyclogenesis compared with wild-type (WT) parasites. 2R metacyclic trypomastigotes had a two-fold increase in resistance to complement mediated lysis and were at least three times more infective to eukaryotic cells, probably due to a higher GP82 expression in the resistant population. Moreover, we have shown that EVs from resistant parasites can transfer the invasive phenotype to the WT population. In addition, we showed that the virulence phenotype of the selected population remains in the trypomastigote form derived from cell culture, which is more infective and also has a higher rate of release of trypomastigotes from infected cells. Altogether, these data indicate that it is possible to select parasites after exposure to a particular stress factor and that the phenotype of epimastigotes remained in the infective stage. Importantly, EVs seem to be an important virulence fator increasing mechanism in this context of survival and persistence in the host. | en_US |
| Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Microbiologia, Parasitologia e Patologia. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências - Bioquímica. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências - Bioquímica. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | School of Human Sciences. London Metropolitan University. London, United Kingdom. / School of Life and Medical Sciences. University of Hertfordshire. London, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Subject | Complement System Proteins | en_US |
| Subject | Chagas Disease | en_US |
| Subject | Extracellular Vesicles | en_US |
| Subject in Spanish | Proteínas del Sistema Complemento | en_US |
| Subject in Spanish | Enfermedad de Chagas | en_US |
| Subject in Spanish | Vesículas Extracelulares | en_US |
| Subject in French | Protéines du système du complément | en_US |
| Subject in French | Maladie de Chagas | en_US |
| Subject in French | Vésicules extracellulaires | en_US |
| DeCS | Trypanosoma cruzi | en_US |
| DeCS | Proteínas do Sistema Complemento | en_US |
| DeCS | Doença de Chagas | en_US |
| DeCS | Vesículas Extracelulares | en_US |
