| Author | Marques, Marlice A. Sípoli | |
| Author | Soares, Alcenir de Souza | |
| Author | Pinto, Olivia Woyames | |
| Author | Barroso, Pedro Tupinambá Werneck | |
| Author | Pinto, Douglas Pereira | |
| Author | Ferreira-Filho, Milton | |
| Author | Werneck-Barroso, Eduardo | |
| Access date | 2023-02-10T20:04:01Z | |
| Available date | 2023-02-10T20:04:01Z | |
| Document date | 2007 | |
| Citation | MARQUES, Marlice A. Sípoli et al. Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: application to pharmacokinetic studies. Journal of Chromatography, v. 852, p. 308–316, 2007. | en_US |
| ISSN | 1570-0232 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57015 | |
| Language | eng | en_US |
| Rights | restricted access | |
| Title | Simple and rapid method determination for metformin in human plasma using high performance liquid chromatography tandem mass spectrometry: application to pharmacokinetic studies | en_US |
| Type | Article | |
| DOI | 10.1016/j.jchromb.2007.01.030 | |
| Abstract | A rapid and simple method for quantitation of metformin (MET) in human plasma by HPLC-MS/MS was developed and validated. The sample preparation consists of plasma deproteinization using acetonitrile. The mobile phase consisted of water-acetonitrile and formic acid (55/45/0.048, v/v/%) and the run time was 3 min. A pursuit C(18) (100 mm x 2.0 mm i.d., 3 microm) column connected to a guard column MS-pursuit (0.20 mm x 0.20 mm i.d., 5 microm) was used. The range of the calibration curve was from 20 to 5000 ng/mL, the limit of quantitation being 20 ng/mL. The detection was performed on a mass spectrometer (ESI+), using metoprolol as internal standard. The calibration curves have r(2) values of 0.995 (CV=0.24%, n=10). The accuracy and precision were between 90.74 and 106.7% and coefficients of variations (CV) of 1.10 and 4.35%, respectively. The method was applied to determine the pharmacokinetic parameters: C(max) (1667.25 ng/mL) and T(max) (3.89 h). | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Instituto de Química. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Cínica Evandro Chagas. Serviço de Farmacocinética. Rio de Janeiro, RJ, Brazil. | |
| Subject | Metformin | en_US |
| Subject | Liquid chromatography | en_US |
| Subject | Mass spectrometry | en_US |
| Subject | Plasma | en_US |
| Subject | Pharmacokinetic | en_US |
| e-ISSN | 1873-376X | |
| Embargo date | 2030-12-31 | |
