| Author | Nicolich, Rebecca S. | |
| Author | Werneck-Barroso, Eduardo | |
| Author | Marques, Marlice A. Sípoli | |
| Access date | 2023-03-07T01:47:51Z | |
| Available date | 2023-03-07T01:47:51Z | |
| Document date | 2006 | |
| Citation | NICOLICH, Rebecca S.; WERNECK-BARROSO, Eduardo; MARQUES, Marlice A. Sípoli. Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, v. 565, p. 97–102, 2006. | en_US |
| ISSN | 0003-2670 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57251 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Title | Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry | en_US |
| Type | Article | |
| DOI | 10.1016/j.aca.2006.01.105 | |
| Abstract | A simple and rapid procedure for extraction of chloramphenicol (CAP) in milk and analysis by high-performance liquid chromatography coupled with quadrupole mass spectrometry in tandem was developed. The method consisted of one step of liquid–liquid extraction using ethyl acetate and acidified water (10 mmol L−1 formic acid) and HPLC-MS/MS detection. CAP-D5 was used as internal standard. The method was validated according to Commission Decision 2002/657/EC. The calibration curves were linear, with typical r2 values higher than 0.98. Absolute recovery of CAP from milk proved to be more than 95%, however CAP-D5 absolute recovery was 75%. The method was accurate and reproducible, being successfully applied to the monitoring of CAP in milk samples obtained from the Brazilian market. Decision limit (CC ) was 0.05 ng mL−1 and detection capability (CC) was 0.09 ng mL−1. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Chloramphenicol | en_US |
| Subject | Antibiotic | en_US |
| Subject | Food control | en_US |
| Subject | High performance liquid chromatography-tandem mass spectrometry | en_US |
| Subject | HPLC-MS/MS | en_US |
| Embargo date | 2030-12-31 | |
