| Author | Sousa, Karina P. de | |
| Author | Rossi, Izadora | |
| Author | Abdullahi, Mahamed | |
| Author | Ramirez, Marcel Ivan | |
| Author | Stratton, Dan | |
| Author | Inal, Jameel Malhador | |
| Access date | 2023-03-15T19:48:33Z | |
| Available date | 2023-03-15T19:48:33Z | |
| Document date | 2023 | |
| Citation | SOUSA, Karina P. de et al. Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomed Nanobiotechnol, v. 15, n. e1835, p. 1–29, 2023. | en_US |
| ISSN | 1939-0041 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57396 | |
| Language | por | en_US |
| Publisher | Wiley | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Isolamento Celular | en_US |
| Title | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy | en_US |
| Type | Article | en_US |
| DOI | 10.1002/wnan.1835 | |
| Abstract | Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. | en_US |
| Affilliation | Bioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK. | en_US |
| Affilliation | School of Human Sciences. London Metropolitan University. London, UK. / Universidade Federal do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | School of Human Sciences. London Metropolitan University. London, UK. | en_US |
| Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Open University. The School of Life, Health, and Chemical Sciences. Milton Keynes, UK. | en_US |
| Affilliation | Bioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK. / School of Human Sciences. London Metropolitan University. London, UK. | en_US |
| Subject | Extracellular Vesicles | en_US |
| Subject | Biomarkers | en_US |
| Subject | Cell Separation | en_US |
| Subject | Pathology, Molecular | en_US |
| Subject in Spanish | Vesículas Extracelulares | en_US |
| Subject in Spanish | Biomarcadores | en_US |
| Subject in Spanish | Separación Celular | en_US |
| Subject in Spanish | Patología Molecular | en_US |
| Subject in French | Vésicules extracellulaires | en_US |
| Subject in French | Marqueurs biologiques | en_US |
| Subject in French | Séparation cellulaire | en_US |
| Subject in French | Anatomopathologie moléculaire | en_US |
| DeCS | Vesículas Extracelulares | en_US |
| DeCS | Biomarcadores | en_US |
| DeCS | Separação Celular | en_US |
| DeCS | Patologia Molecular | en_US |
