| Author | Diniz Filho, Joel Félix Silva | |
| Author | Barros, Aline Oliveira da Silva de | |
| Author | Pijeira, Martha Sahylí Ortega | |
| Author | Ricci-Junior, Eduardo | |
| Author | Midlej, Victor | |
| Author | Baroni, Mariana Pelissari Monteiro Aguiar | |
| Author | Santos, Clenilton Costa dos | |
| Author | Alencar, Luciana Magalhães Rebelo | |
| Author | Oliveira, Ralph Santos | |
| Access date | 2023-03-31T19:09:37Z | |
| Available date | 2023-03-31T19:09:37Z | |
| Document date | 2023 | |
| Citation | DINIZ FILHO, Joel Félix Silva et al. Ultrastructural Analysis of Cancer Cells Treated with the Radiopharmaceutical Radium Dichloride ([223Ra]RaCl2): Understanding the Effect on Cell Structure. Cells, v. 12, 451,p. 1 - 12, Jan. 2023. | en_US |
| ISSN | 2073-4409 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57646 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Morte celular | en_US |
| Subject in Portuguese | Mecanismo celular | en_US |
| Subject in Portuguese | Terapia alfa direcionada | en_US |
| Subject in Portuguese | Radiofármaco | en_US |
| Title | Ultrastructural Analysis of Cancer Cells Treated with the Radiopharmaceutical Radium Dichloride ([223Ra]RaCl2): Understanding the Effect on Cell Structure | en_US |
| Type | Article | |
| DOI | 10.3390/ cells12030451 | |
| Abstract | The use of alpha-particle ( -particle) radionuclides, especially [223Ra]RaCl2 (radium dichloride),
for targeted alpha therapy is steadily increasing. Despite the positive clinical outcomes of this
therapy, very little data are available about the effect on the ultrastructure of cells. The purpose of this
study was to evaluate the nanomechanical and ultrastructure effect of [223Ra] RaCl2 on cancer cells.
To analyze the effect of [223Ra]RaCl2 on tumor cells, human breast cancer cells (lineage MDA-MB-231)
were cultured and treated with the radiopharmaceutical at doses of 2 Ci and 0.9 Ci. The effect
was evaluated using atomic force microscopy (AFM) and transmission electron microscopy (TEM)
combined with Raman spectroscopy. The results showed massive destruction of the cell membrane
but preservation of the nucleus membrane. No evidence of DNA alteration was observed. The data
demonstrated the formation of lysosomes and phagosomes. These findings help elucidate the main
mechanism involved in cell death during -particle therapy. | en_US |
| Affilliation | Universidade Federal do Maranhão. Departamento de Física. Laboratório de Biofísica e Nanossistemas. São Luis, MA, Brasil. | en_US |
| Affilliation | Comissão Brasileira. Instituto de Engenharia Nuclear. Laboratório de Nanoradiofarmácia e Síntese de Novos Radiofármacos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Comissão Brasileira. Instituto de Engenharia Nuclear. Laboratório de Nanoradiofarmácia e Síntese de Novos Radiofármacos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Escola de Farmácia. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Instituto Federal de Educação, Ciência e Tecnologia. Departamento de Ciências Naturais e Matemática. Campus São Paulo, São Paulo, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Maranhão. Departamento de Física. Laboratório de Biofísica e Nanossistemas. São Luis, MA, Brasil. | en_US |
| Affilliation | Universidade Federal do Maranhão. Departamento de Física. Laboratório de Biofísica e Nanossistemas. São Luis, MA, Brasil. | en_US |
| Affilliation | Comissão Brasileira. Instituto de Engenharia Nuclear. Laboratório de Nanoradiofarmácia e Síntese de Novos Radiofármacos. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro, Laboratório de Radiofarmácia e Nanorradiofármacos. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Cell death | en_US |
| Subject | Cell mechanism | en_US |
| Subject | Targeted alpha therapy | en_US |
| Subject | Radiopharmaceutical | en_US |
