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AutorGarcia-Princival IMR
AutorPrincival JL
AutorDias da Silva E
Autorde Arruda Lima SM
AutorCarregosa JC
AutorWisniewski A Jr
Autorde Lucena CCO
AutorHalwass F
AutorAlves Franca JA
AutorFerreira LFGR
AutorHernandes MZ
AutorSaraiva KLA
AutorPeixoto CA
AutorBaratte B
AutorRobert T
AutorBach S
AutorGomes DC
AutorGuedes Paiva PM
AutorMarchand P
AutorRodrigues MDD
AutorGonçalves da Silva T.
Data de acesso2023-04-10T14:26:59Z
Data de disponibilização2023-04-10T14:26:59Z
Data do publicação2021
CitaçãoGARCIA-PRINCIVAL, Iza Mirela Rodini; PRINCIVAL, Jefferson Luiz; SILVA, Emmanuel Dias da; LIMA, Sandrine Maria de Arruda; CARREGOSA, Jhonattas Carvalho; WISNIEWSKI JUNIOR, Alberto; LUCENA, Caio Cézar Oliveira de; HALWASS, Fernando; FRANCA, José Adonias Alves; FERREIRA, Luiz Felipe Gomes Rebello. Streptomyces hygroscopicus UFPEDA 3370: a valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells. Chemico-Biological Interactions, [S.L.], v. 333, p. 109316, jan. 2021. Elsevier BV. http://dx.doi.org/10.1016/j.cbi.2020.109316.en_US
ISSN1872-7786en_US
URIhttps://www.arca.fiocruz.br/handle/icict/57724
Idiomaporen_US
Direito Autoralopen accessen_US
TítuloStreptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cellsen_US
Tipo do documentoArticleen_US
DOI10.1016/j.cbi.2020.109316
Resumo em InglêsStreptomyces hygroscopicus UFPEDA 3370 was fermented in submerged cultivation and the biomass extract was partitioned, obtaining a fraction purified named EB1. After purification of EB1 fraction, nigericin free acid was obtained and identified. Nigericin presented cytotoxic activity against several cancer cell lines, being most active against HL-60 (human leukemia) and HCT-116 (human colon carcinoma) cell lines, presenting IC50 and (IS) values: 0.0014 μM, (30.0) and 0.0138 μM (3.0), respectively. On HCT-116, nigericin caused apoptosis and autophagy. In this study, nigericin was also screened both in vitro and in silico against a panel of cancer-related kinases. Nigericin was able to inhibit both JAK3 and GSK-3β kinases in vitro and its binding affinities were mapped through the intermolecular interactions with each target in silico.en_US
AfiliaçãoFundação Oswaldo Cruz, Instituto Aggeu Magalhães, Laboratório de Ultraestrutura, Recife, PE, Brazil. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Universidade Federal de Pernambuco, Departamento de Química Fundamental, Recife, Pernambuco, Brasil. Universidade Federal de Sergipe, Departamento de Química, Aracaju, Sergipe, Brasil. Universidade Federal de Pernambuco, Departamento de Ciências Farmacêuticas, Laboratório de Química Teórica Medicinal (LQTM), Recife, PE, Brazil. Instituto de Ciências e Tecnologia em Neuroimunomodulação (INCT-NIM) Universidade Federal de Pernambuco, Departamento de Bioquímica, Recife, Pernambuco, Brasil. Universite de Nantes, Cibles et médicaments des infections et du cancer, IICiMed, EA 1155, Nantes, F-44000, France. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic address: baratte@sb-roscoff.fr. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France.
Palavras-chave em inglêsAutophagyen_US
Palavras-chave em inglêsNatural productsen_US
Palavras-chave em inglêsNigericinen_US
Palavras-chave em inglêsProtein kinasesen_US
Palavras-chave em inglêsApoptosisen_US


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