| Author | Ataides, Laura Sant’Anna | |
| Author | Maia, Fernanda de Moraes | |
| Author | Conte, Fernando Paiva | |
| Author | Isaac, Lourdes | |
| Author | Barbosa, Angela Silva | |
| Author | Lima Junior, Josué da Costa | |
| Author | Avelar, Kátia Eliane Santos | |
| Author | Silva, Rodrigo Nunes Rodrigues | |
| Access date | 2023-04-13T18:33:46Z | |
| Available date | 2023-04-13T18:33:46Z | |
| Document date | 2023 | |
| Citation | ATAIDES, Laura Sant`Anna et al. Sph2(176–191) and Sph2(446–459): Identification of B-Cell Linear Epitopes in Sphingomyelinase 2 (Sph2), Naturally Recognized by Patients Infected by Pathogenic Leptospires. Vaccines, v. 11, 359, p. 1 - 19, Feb. 2023. | en_US |
| ISSN | 2076-393X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57768 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Leptospirose | en_US |
| Subject in Portuguese | Epítopo de células B | en_US |
| Subject in Portuguese | Imunoinformática | en_US |
| Title | Sph2(176–191) and Sph2(446–459): Identification of B-Cell Linear Epitopes in Sphingomyelinase 2 (Sph2), Naturally Recognized by Patients Infected by Pathogenic Leptospires | en_US |
| Type | Article | |
| DOI | 10.3390/ vaccines11020359 | |
| Abstract | Sphingomyelin is a major constituent of eukaryotic cell membranes, and if degraded by
bacteria sphingomyelinases may contribute to the pathogenesis of infection. Among Leptospira spp.,
there are five sphingomyelinases exclusively expressed by pathogenic leptospires, in which Sph2
is expressed during natural infections, cytotoxic, and implicated in the leptospirosis hemorrhagic
complications. Considering this and the lack of information about associations between Sph2 and
leptospirosis severity, we use a combination of immunoinformatics approaches to identify its B-cell
epitopes, evaluate their reactivity against samples from leptospirosis patients, and investigate the role
of antibodies anti-Sph2 in protection against severe leptospirosis. Two B-cell epitopes, Sph2(176-191)
and Sph2(446-459), were predicted in Sph2 from L. interrogans serovar Lai, presenting different levels
of identity when compared with other pathogenic leptospires. These epitopes were recognized by
about 40% of studied patients with a prevalence of IgG antibodies against both Sph2(176-191) and
Sph2(446-459). Remarkably, just individuals with low reactivity to Sph2(176-191) presented clinical
complications, while high responders had only mild symptoms. Therefore, we identified two B-cell
linear epitopes, recognized by antibodies of patients with leptospirosis, that could be further explored
in the development of multi-epitope vaccines against leptospirosis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório Piloto Eucariotos. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil. | en_US |
| Affilliation | Instituto Butantan. Laboratório de Bacteriologia. São Paulo, SP, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional para Leptospirose. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Leptospirosis | en_US |
| Subject | B-cell epitope | en_US |
| Subject | Immunoinformatic | en_US |
