| Author | Pinto, Priscila de Faria | |
| Author | Montesano, Maria Ângela | |
| Author | Jacinto, Alexandre Arthur | |
| Author | Santos, Ronaldo Soares | |
| Author | Bordin, Fábio Humberto S. | |
| Author | Ferreira, Ana Paula | |
| Author | Penido, Marcus Luiz de Oliveira | |
| Author | Coelho, Paulo Marcos Zach | |
| Author | Vasconcelos, Eveline Gomes | |
| Access date | 2012-11-12T13:56:10Z | |
| Available date | 2012-11-12T13:56:10Z | |
| Document date | 2010 | |
| Citation | PINTO, Priscila de Faria et al. Antibody reactivity against potato apyrase, a protein that shares epitopes with Schistosoma mansoni ATP diphosphohydrolase isoforms, in acute and chronically infected mice, after chemotherapy and reinfection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 4, p. 374-379, 2010. | pt_BR |
| ISSN | 0074-0276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/5787 | |
| Sponsorship | Fapeapeapemigig, CNPq, CPqRR (to PFP and AAJ), PIBIC, PROBIC/UFJF (to RSS and FHSB | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Title | Antibody reactivity against potato apyrase, a protein that shares epitopes with Schistosoma mansoni ATP diphosphohydrolase isoforms, in acute and chronically infected mice, after chemotherapy and reinfection | pt_BR |
| Type | Article | pt_BR |
| Abstract | Schistosoma mansoni ATP diphosphohydrolase isoforms and potato apyrase share conserved epitopes. By en¬zyme-linked immunosorbent assays, elevated levels of IgM, IgG2a and IgG1 antibody reactivity against potato apy¬rase were observed in S. mansoni-infected BALB/c mice during the acute phase of infection, while only IgM and IgG1 antibody reactivity levels maintained elevated during the chronic phase of infection. Antibody reactivity against po¬tato apyrase was monitored over an 11-month period in chronically-infected mice treated with oxamniquine. Eleven months later, the level of seropositive IgM decreased significantly (~30%) compared to the level found in untreated, infected mice. The level of seropositive IgG1 decreased significantly four months after treatment (MAT) (61%) and remained at this level even after 11 months. The IgG2a reactivity against potato apyrase, although unchanged during chronic phase to 11 MAT, appeared elevated again in re-infected mice suggesting a response similar to that found during the acute phase. BALB/c mouse polyclonal anti-potato apyrase IgG reacted with soluble egg antigens prob¬ably due to the recognition of parasite ATP diphosphohydrolase. This study, for the first time, showed that the IgG2a antibody from S. mansoni-infected BALB mice cross-reacts with potato apyrase and the level of IgG2a in infected mice differentiates disease phases. The results also suggest that different conserved-epitopes contribute to the im¬mune response in schistosomiasis. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas . Departamento de Bioquímica. Juiz de Fora, MG, Brasil / Santa Casa de Belo Horizonte. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Santa Casa de Belo Horizonte. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas . Departamento de Bioquímica. Juiz de Fora, MG, Brasil / Santa Casa de Belo Horizonte. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | ATP diphosphohydrolase | pt_BR |
| Subject | Potato apyrase | pt_BR |
| Subject | Antibody - BALB/c mouse | pt_BR |
| Subject | Chemotherapy | pt_BR |
