| Author | Beltrame, Jeovandro Maria | |
| Author | Ribeiro, Brena Beatriz Pereira | |
| Author | Guindani, Camila | |
| Author | Candiotto, Graziâni | |
| Author | Felipe, Karina Bettega | |
| Author | Lucas, Rodrigo | |
| Author | Zottis, Alexandre D’Agostini | |
| Author | Isoppo, Eduardo | |
| Author | Sayer, Claudia | |
| Author | Araújo, Pedro Henrique Hermes de | |
| Access date | 2023-04-19T11:53:55Z | |
| Available date | 2023-04-19T11:53:55Z | |
| Document date | 2023 | |
| Citation | BELTRAME, Jeovandro Maria et al. Coating of SPIONs with a cysteine-decorated copolyester: A possible novel nanoplatform for enzymatic release. Pharmaceutics, v. 15, n. 1000, p. 1-15, 2023. | en_US |
| ISSN | 1999-4923 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57887 | |
| Language | por | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Coating of SPIONs with a cysteine-decorated copolyester: A possible novel nanoplatform for enzymatic release | en_US |
| Type | Article | en_US |
| DOI | https://doi.org/ 10.3390/pharmaceutics15031000 | |
| Abstract | Superparamagnetic iron oxide nanoparticles (SPIONs) have their use approved for the diagnosis/treatment of malignant tumors and can be metabolized by the organism. To prevent embolism caused by these nanoparticles, they need to be coated with biocompatible and non-cytotoxic materials. Here, we synthesized an unsaturated and biocompatible copolyester, poly (globalideco-ε-caprolactone) (PGlCL), and modified it with the amino acid cysteine (Cys) via a thiol-ene reaction (PGlCLCys). The Cys-modified copolymer presented reduced crystallinity and increased hydrophilicity in comparison to PGlCL, thus being used for the coating of SPIONS (SPION@PGlCLCys). Additionally, cysteine pendant groups at the particle’s surface allowed the direct conjugation of (bio)molecules that establish specific interactions with tumor cells (MDA-MB 231). The conjugation of either folic acid (FA) or the anti-cancer drug methotrexate (MTX) was carried out directly on the amine groups of cysteine molecules present in the SPION@PGlCLCys surface (SPION@PGlCLCys_FA and SPION@PGlCLCys_MTX) by carbodiimide-mediated coupling, leading to the formation of amide bonds, with conjugation efficiencies of 62% for FA and 60% for MTX. Then, the release of MTX from the nanoparticle surface was evaluated using a protease at 37 ◦C in phosphate buffer pH~5.3. It was found that 45% of MTX conjugated to the SPIONs were released after 72 h. Cell viability was measured by MTT assay, and after 72 h, 25% reduction in cell viability of tumor cells was observed. Thus, after a successful conjugation and subsequent triggered release of MTX, we understand that SPION@PGlCLCys has a strong potential to be treated as a model nanoplatform for the development of treatments and diagnosis techniques (or theranostic applications) that can be less aggressive to patients. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Engenharia Química e Engenharia de Alimentos. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Engenharia Química e Engenharia de Alimentos. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. COPPE. Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Física. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Análises Clínicas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Instituto Federal de Santa Catarina. Departamento Acadêmico de Saúde e Serviços. NANOTEC Group. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório Central de Microscopia Eletrônica. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Engenharia Química e Engenharia de Alimentos. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Engenharia Química e Engenharia de Alimentos. Florianópolis, SC, Brasil. | en_US |
| Subject | Magnetic Iron Oxide Nanoparticles | en_US |
| Subject | Cysteine | en_US |
| Subject | Cytotoxins | en_US |
| Subject | Coated Materials, Biocompatible | en_US |
| Subject | Theranostic Nanomedicine | en_US |
| Subject in Spanish | Nanopartículas Magnéticas de Óxido de Hierro | en_US |
| Subject in Spanish | Cisteína | en_US |
| Subject in Spanish | Citotoxinas | en_US |
| Subject in Spanish | Materiales Biocompatibles Revestidos | en_US |
| Subject in Spanish | Nanomedicina Teranóstica | en_US |
| Subject in French | Nanoparticules magnétiques d'oxyde de fer | en_US |
| Subject in French | Cystéine | en_US |
| Subject in French | Cytotoxines | en_US |
| Subject in French | Matériaux revêtus, biocompatibles | en_US |
| Subject in French | Nanomédecine théranostique | en_US |
| DeCS | Nanopartículas Magnéticas de Óxido de Ferro | en_US |
| DeCS | Cisteína | en_US |
| DeCS | Citotoxinas | en_US |
| DeCS | Materiais Revestidos Biocompatíveis | en_US |
| DeCS | Nanomedicina Teranóstica | en_US |
