| Author | Cherkaoui, Dounia | |
| Author | Mesquita, Silvia Gonçalves | |
| Author | Huang, Da | |
| Author | Lugli, Elena B | |
| Author | Webster, Bonnie L | |
| Author | McKendry, Rachel A | |
| Access date | 2023-04-19T17:58:10Z | |
| Available date | 2023-04-19T17:58:10Z | |
| Document date | 2023 | |
| Citation | CHERKAOUI, Dounia et al. CRISPR-assisted test for Schistosoma haematobium. Sci Rep, 13, n. 1, 4990, 2023. doi: 10.1038/s41598-023-31238-y. | en_US |
| ISSN | 2045-2322 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57892 | |
| Language | eng | en_US |
| Publisher | Nature Publishing Group | en_US |
| Rights | open access | en_US |
| Title | CRISPR-assisted test for Schistosoma haematobium | en_US |
| Type | Article | |
| Abstract | Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2 h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases. | en_US |
| Affilliation | London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK. | en_US |
| Affilliation | Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK. | en_US |
| Affilliation | London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK. | en_US |
| Affilliation | Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK. | en_US |
| Affilliation | Wolfson Wellcome Biomedical Laboratories. Department of Science. Natural History Museum. Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK. | en_US |
| Affilliation | London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK. | en_US |
