Author | Lima, Lienne D'Auria | |
Author | Reis, Adriana Cotta Cardoso | |
Author | Sousa, Jordano Augusto Carvalho | |
Author | Valente, Gabriel Mendonça | |
Author | Silva, Breno de Mello | |
Author | Magalhães, Cíntia Lopes de Brito | |
Author | Kohlhoff, Markus | |
Author | Teixeira, Luiz Fernando de Medeiros | |
Author | Brandão, Geraldo Célio | |
Access date | 2023-04-24T17:00:45Z | |
Available date | 2023-04-24T17:00:45Z | |
Document date | 2023 | |
Citation | LIMA, Lienne D'Auria et al. Anti-Zika Virus Activity and Isolation of Flavonoids from Ethanol Extracts of Curatella americana L. Leaves. Molecules, v. 28, n. 6, 2546, 2023. doi: 10.3390/molecules28062546. | en_US |
ISSN | 1420-3049 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/57940 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Anti-Zika Virus Activity and Isolation of Flavonoids from Ethanol Extracts of Curatella americana L. Leaves | en_US |
Type | Article | en_US |
Abstract | The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian Cerrado, whose ethanolic extract showed significant in vitro anti-Zika virus activity by the MTT colorimetric method. Currently, there is no drug in clinical use specifically for the treatment of this virus; therefore, in this work, the antiviral and cytotoxic properties of the ethanolic extract, fractions, and compounds were evaluated. The ethanolic extract of the leaves showed no cytotoxicity for the human MRC-5 cell and was moderately cytotoxic for the Vero cell (CC50 161.5 ± 2.01 µg/mL). This extract inhibited the Zika virus multiplication cycle with an EC50 of 85.2 ± 1.65 µg/mL. This extract was fractionated using the liquid-liquid partition technique, and the ethyl acetate fraction showed significant activity against the Zika virus with an EC50 of 40.7 ± 2.33 µg/mL. From the ethyl acetate fraction, the flavonoids quercetin-3-O-hexosylgallate (1), quercetin-3-O-glucoside (2), and quercetin (5) were isolated, and in addition to these compounds, a mixture of quercetin-3-O-rhamnoside (3) and quercetin-3-O-arabinoside (4) was also obtained. The isolated compounds quercetin and quercetin-3-O-hexosylgallate inhibited the viral cytopathic effect at an EC50 of 18.6 ± 2.8 and 152.8 ± 2.0, respectively. Additionally, analyses by liquid chromatography coupled to a mass spectrometer allowed the identification of another 24 minor phenolic constituents present in the ethanolic extract and in the ethyl acetate fraction of this species. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto, MG, Brazil. | en_US |
Subject | Curatella americana | en_US |
Subject | Zika virus | en_US |
Subject | cytotoxicity | en_US |
Subject | flavonoid | en_US |
Subject | phytochemistry | en_US |