| Author | Logullo, Jorgete | |
| Author | LIma, Israel Diniz | |
| Author | Rocha, Juliana Dutra B. | |
| Author | Cortê-Real, Suzana | |
| Author | Silva-Júnior, Elias Barbosa da | |
| Author | Oliveira, Joyce Cristina Guimarães de | |
| Author | Morrot, Alexandre | |
| Author | Fonseca, Leonardo Marques da | |
| Author | Lima, Leonardo Freire de | |
| Author | Decote-Ricardo, Debora | |
| Author | Lima, Celio Geraldo Freire de | |
| Access date | 2023-04-29T14:28:58Z | |
| Available date | 2023-04-29T14:28:58Z | |
| Document date | 2023 | |
| Citation | LOGULLO, Jorgete et al. Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix. Life, v.13, 1063, p. 11 - 18, Apr. 2023. | en_US |
| ISSN | 2075-1729 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58083 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | Matriz extracelular | en_US |
| Subject in Portuguese | Macrófagos | en_US |
| Subject in Portuguese | Trypanosoma cruzi | en_US |
| Subject in Portuguese | Colágeno I | en_US |
| Subject in Portuguese | Matriz 3D | en_US |
| Title | Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix | en_US |
| Type | Article | |
| DOI | 10.3390/life13041063 | |
| Abstract | The interactions between cell and cellular matrix confers plasticity to each body tissue,
influencing the cellular migratory capacity. Macrophages rely on motility to promote their physiological
function. These phagocytes are determinant for the control of invasive infections, and their
immunological role largely depends on their ability to migrate and adhere to tissue. Therefore, they
interact with the components of the extracellular matrix through their adhesion receptors, conferring
morphological modifications that change their shape during migration. Nevertheless, the need to
use in vitro cell growth models with the conditioning of three-dimensional synthetic matrices to
mimic the dynamics of cell-matrix interaction has been increasingly studied. This becomes more
important to effectively understand the changes occurring in phagocyte morphology in the context of
infection progression, such as in Chagas disease. This disease is caused by the intracellular pathogen
Trypanosoma cruzi, capable of infecting macrophages, determinant cells in the anti-trypanosomatid
immunity. In the present study, we sought to understand how an in vitro extracellular matrix model
interferes with T. cruzi infection in macrophages. Using different time intervals and parasite ratios,
we evaluated the cell morphology and parasite replication rate in the presence of 3D collagen I matrix.
Nevertheless, microscopy techniques such as scanning electron microscopy were crucial to trace
macrophage-matrix interactions. In the present work, we demonstrated for the first time that the
macrophage-matrix interaction favors T. cruzi in vitro replication and the release of anti-inflammatory
cytokines during macrophage infection, in addition to drastically altering the morphology of the
macrophages and promoting the formation of migratory macrophages. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitogia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Extracellular matrix | en_US |
| Subject | Macrophages | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Collagen I | en_US |
| Subject | 3D matrix | en_US |
