| Author | Machado, Hellen Cristine | |
| Author | Bispo, Saloe | |
| Author | Dallagiovanna, Bruno | |
| Access date | 2023-05-09T19:30:32Z | |
| Available date | 2023-05-09T19:30:32Z | |
| Document date | 2023 | |
| Citation | MACHADO, Hellen Cristine et al. miR-6087 might regulate cell cycle–related mRNAs during cardiomyogenesis of hESCs. Bioinformatics and Biology Insights, v. 17, p. 1-11, 2023. | en_US |
| ISSN | 1177-9322 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58227 | |
| Language | por | en_US |
| Publisher | SAGE Publications | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | miR-6087 | en_US |
| Title | miR-6087 might regulate cell cycle–related mRNAs during cardiomyogenesis of hESCs | en_US |
| Type | Article | en_US |
| DOI | https://doi.org/10.1177/11779322231161918 | |
| Abstract | MicroRNAs (miRNAs) are small noncoding RNAs that act as negative regulators of gene expression at the post-transcriptional level, promoting mRNA degradation or translation repression. Despite the well-described presence of miRNAs in various human tissues, there is still a lack of information about the relationship between miRNAs and the translation regulation in human embryonic stem cells (hESCs) during cardiomyogenesis. Here, we investigate RNA-seq data from hESCs, focusing on distinct stages of cardiomyogenesis and searching for polysome-bound miRNAs that could be involved in translational regulation. We identify miR-6087 as a differentially expressed miRNA at latest steps of cardiomyocyte differentiation. We analyzed the coexpression pattern between the differentially expressed mRNAs and miR-6087, evaluating whether they are predicted targets of the miRNA. We arranged the genes into an interaction network and identified BLM, RFC4, RFC3, and CCNA2 as key genes of the network. A post hoc analysis of the key genes suggests that miR-6087 could act as a regulator of the cell cycle in hESC during cardiomyogenesis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
| Subject | polysome | en_US |
| Subject | Cell Cycle | en_US |
| Subject | Gene Regulatory Networks | en_US |
| Subject in Spanish | Ciclo Celular | en_US |
| Subject in Spanish | Redes Reguladoras de Genes | en_US |
| Subject in French | Cycle cellulaire | en_US |
| Subject in French | Réseaux de régulation génique | en_US |
| DeCS | Polissomos | en_US |
| DeCS | Ciclo Celular | en_US |
| DeCS | RNA-Seq | en_US |
| DeCS | Redes Reguladoras de Genes | en_US |
