| Author | Andrade, Renata Aline de | |
| Author | Filho, Olindo Assis Martins | |
| Author | Araújo, Márcio Sobreira Silva | |
| Author | Reis, Alexandre Barbosa | |
| Author | Gontijo, Célia Maria Ferreira | |
| Author | Vianna, Leonardo Rocha | |
| Author | Mayrink, Wilson | |
| Author | Martins Filho, Olindo Assis | |
| Access date | 2023-05-12T16:21:52Z | |
| Available date | 2023-05-12T16:21:52Z | |
| Document date | 2009 | |
| Citation | ANDRADE, Renata Aline et al. Advances in flow cytometric serology for canine visceral leishmaniasis: diagnostic applications when distinct clinical forms, vaccination and other canine pathogens become a challenge. Vet Immunol Immunopathol., v. 128, n. 1-3, p. 79-86, 2009. doi: 10.1016/j.vetimm.2008.10.308. | en_US |
| ISSN | 0165-2427 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58355 | |
| Language | eng | en_US |
| Publisher | Elsevier Scientific | en_US |
| Rights | restricted access | |
| Title | Advances in flow cytometric serology for canine visceral leishmaniasis: Diagnostic applications when distinct clinical forms, vaccination and other canine pathogens become a challenge | en_US |
| Type | Article | |
| DOI | 10.1016/j.vetimm.2008.10.308 | |
| Abstract | We have previously reported the applicability of flow cytometry anti-fixed Leishmania infantum chagasi promastigotes IgG (FC-AFPA-IgG) as a novel serological device for laboratorial diagnosis of CVL. Herein, we validate throughout a blind study applied into a broader range of coded sera samples that FC-AFPA-IgG at serum dilution 1:8192 have an outstanding performance to discriminate the serological reactivity of canine visceral leishmaniasis (CVL, n = 64) and Leishmune (R) vaccines (VAC, n = 62) and non-infected controls (NI, n = 25). Moreover, we have evaluated the performance of indirect immunofluorescence antibody test (IFAT) and the crude-antigen enzyme-linked immunosorbent assay (ELISA) in parallel with FC-AFPA-IgG, to discriminate the seroreactivity of NI, CVL and VAC. Our data demonstrated that both ELISA and FC-AFPA-IgG showed similar performance to detect the seronegativity in 100% of NI, whereas FC-AFPA-IgG displayed better performance to exclude seropositivity in 100% of VAC. The high kappa agreement indexes observed suggested similar performance between these two serological testes when distinct clinical forms of CVL become a challenge. Furthermore, the FC-AFPA-IgG applied at sera dilution 1:8192 showed a remarkable performance to discriminate CVL from other co-endemic canine infections with high co-negativity in dogs infected with Trypanosoma cruzi and Leishmania braziliensis (86% and 84%, respectively). In conclusion, the data presented here re-emphasize the applicability of FC-AFPA-IgG as an innovative methodology able to discriminate post-infection imunomediated seroreactivity from that triggered by prophylactic immunization with minor cross-reactivity with other relevant canine pathogens, which may contribute as a supplementary assay for the CVL immunodiagnosis. (C) 2008 Elsevier B.V. All rights reserved | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Escola de Farmácia Departamento de Análises Clínicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Estado de Minas Gerais. Polícia Militar de Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | en_US |
| Subject | Flow cytometry | en_US |
| Subject | FC-AFPA-IgG | en_US |
| Subject | IFAT | en_US |
| Subject | ELISA | en_US |
| Subject | Canine visceral leishmaniasis | en_US |
| Subject | Leishmania infantum chagasi | en_US |
| Subject | Leishmune (R) | en_US |
