| Author | Santos, Cristiano C. P. dos | |
| Author | Frézard, Frédéric | |
| Author | Ramos, Guilherme Santos | |
| Author | Paula, Renata C De | |
| Author | Faria, Karen F | |
| Author | Moreira, Paulo Otávio Lourenço | |
| Author | Pereira, Ramon A | |
| Author | Melo, Maria Norma | |
| Author | Tafuri, Wagner Luiz | |
| Author | Demicheli, Cynthia | |
| Author | Ribeiro, Raul Rio | |
| Author | Azevedo, Erly Guilherme | |
| Author | Monte Neto, Rubens Lima Do | |
| Author | Silva, Sydnei Magno da | |
| Author | Frézard, Frédéric | |
| Access date | 2023-05-15T15:18:20Z | |
| Available date | 2023-05-15T15:18:20Z | |
| Document date | 2020 | |
| Citation | SANTOS Cristiano C P Dos, et al. Therapeutic Efficacy of a Mixed Formulation of Conventional and PEGylated Liposomes Containing Meglumine Antimoniate, Combined with Allopurinol, in Dogs Naturally Infected with Leishmania infantum. Antimicrob Agents Chemother., v. 64, n. 7, e00234-20, 2020. doi: 10.1128/AAC.00234-20. | en_US |
| ISSN | 0066-4804 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58391 | |
| Language | eng | en_US |
| Publisher | American Society for Microbiology | en_US |
| Rights | restricted access | |
| Title | Therapeutic Efficacy of a Mixed Formulation of Conventional and PEGylated Liposomes Containing Meglumine Antimoniate, Combined with Allopurinol, in Dogs Naturally Infected with Leishmania infantum | en_US |
| Type | Article | |
| DOI | 10.1128/AAC.00234-20 | |
| Abstract | The treatment of dogs naturally infected with Leishmania infantum using meglumine antimoniate (MA) encapsulated in conventional liposomes (LC) in association with allopurinol has been previously reported to promote a marked reduction in the parasite burden in the main infection sites. Here, a new assay in naturally infected dogs was performed using a novel liposome formulation of MA consisting of a mixture of conventional and long-circulating (PEGylated) liposomes (LCP), with expected broader distribution among affected tissues of the mononuclear phagocyte system. Experimental groups of naturally infected dogs were as follows: LCP plus Allop, receiving LCP intravenously as 2 cycles of 6 doses (6.5 mg Sb/kg of body weight/dose) at 4-day intervals plus allopurinol at 30 mg/kg/12 h per os (p.o.) during 130 days (LCP+Allop); LC plus Allop, receiving LC intravenously as 2 cycles of 6 doses (6.5 mg Sb/kg/dose) plus allopurinol during 130 days (LC+Allop); Allop, treated with allopurinol only; and a nontreated control. Parasite loads were evaluated by quantitative PCR in liver, spleen, and bone marrow tissue and by immunohistochemistry in the ear skin, before treatment, just after treatment, and 4 months later. The LCP+Allop and LC+Allop groups, but not the Allop group, showed significant suppression of the parasites in the liver, spleen, and bone marrow 4 months after treatment compared to the pretreatment period or the control group. Only LCP+Allop group showed significantly lower parasite burden in the skin in comparison to the control group. On the basis of clinical staging and parasitological evaluations, the LCP formulation exhibited a more favorable therapeutic profile than the LC one, being therefore promising for the treatment of canine visceral leishmaniasis. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Química. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Medicina Veterinária. Juiz de Fora, Minas Gerais, Brazil. | en_US |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Farmácia. Governador Valadares, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brazil | en_US |
| Subject | PEG | en_US |
| Subject | allopurinol | en_US |
| Subject | antimony | en_US |
| Subject | dogs | en_US |
| Subject | drug delivery | en_US |
| Subject | drug resistance | en_US |
| Subject | liposomes | en_US |
| Subject | meglumine antimoniate | en_US |
| Subject | visceral leishmaniasis | en_US |
| Embargo date | 2030-12-31 | |
