| Author | Queiroz, Fábio Ribeiro | |
| Author | Portilho, Laysa Gomes | |
| Author | Jeremias, Wander de Jesus | |
| Author | Babá, Élio Hideo | |
| Author | Amaral, Laurence Rodrigues do | |
| Author | Silva, Luciana Maria | |
| Author | Coelho, Paulo Marcos Zech | |
| Author | Caldeira, Roberta Lima | |
| Author | Gomes, Matheus de Souza | |
| Access date | 2023-05-19T13:19:24Z | |
| Available date | 2023-05-19T13:19:24Z | |
| Document date | 2020 | |
| Citation | QUEIROZ, Fábio Ribeiro et al. Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata. Mem Inst Oswaldo Cruz, v. 115, e190498, 2020. doi: 10.1590/0074-02760190498. | en_US |
| ISSN | 0074-0276 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58497 | |
| Language | eng | en_US |
| Publisher | Instituto Oswaldo Cruz | en_US |
| Rights | open access | en_US |
| Title | Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata | en_US |
| Type | Article | |
| DOI | 10.1590/0074-02760190498 | |
| Abstract | BACKGROUND Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs have become more relevant. The small RNAs have been considered important through their ability to finely regulate the gene expression in several organisms, thus controlling the functions like cell growth, metabolism, and susceptibility/resistance to infection. OBJECTIVE The present study aims on identification and characterisation of the repertoire of small non-coding RNAs in B. glabrata (Bgl-small RNAs). METHODS By using small RNA sequencing, bioinformatics tools and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we identified, characterised, and validated the presence of small RNAs in B. glabrata. FINDINGS 89 mature miRNAs were identified and five of them were classified as Mollusk-specific. When compared to model organisms, sequences of B. glabrata miRNAs showed a high degree of conservation. In addition, several target genes were predicted for all the mature miRNAs identified. Furthermore, piRNAs were identified in the genome of B. glabrata for the first time. The B. glabrata piRNAs showed strong conservation of uridine as first nucleotide at 5' end, besides adenine at 10th position. Our results showed that B. glabrata has diverse repertoire of circulating ncRNAs, several which might be involved in mollusk susceptibility to infection, due to their potential roles in the regulation of S. mansoni development. MAIN CONCLUSIONS Further studies are necessary in order to confirm the role of the Bgl-small RNAs in the parasite/host relationship thus opening new perspectives on interference of small RNAs in the organism development and susceptibility to infection. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Patos de Minas, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil/Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Patos de Minas, MG, Brasil. | en_US |
| Affilliation | Fundação Ezequiel Dias. Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil/Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Patos de Minas, MG, Brasil. | en_US |
| Subject | schistosomiasis | en_US |
| Subject | non-coding RNAs | en_US |
| Subject | mollusk vectors | en_US |
| Subject | next-generation sequencing | en_US |
