| Author | Gomes, Juliana Moreira Mendonça | |
| Author | Charlie-Silva, Ives | |
| Author | Santos, Anderson Kenedy | |
| Author | Rezende, Rodrigo Ribeiro | |
| Author | Estanislau, Juliana de Assis Silva Gomes | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Corrêa Junior, José Dias | |
| Access date | 2023-05-23T12:54:27Z | |
| Available date | 2023-05-23T12:54:27Z | |
| Document date | 2020 | |
| Citation | GOMES, Juliana Moreira Mendonça et al. An imaging flow cytometry-based technique to quantify erythrocyte nuclear alterations. Aquat Toxicol., v. 228, 105649, 2020. doi: 10.1016/j.aquatox.2020.105649. | en_US |
| ISSN | 0166-445X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58574 | |
| Language | eng | en_US |
| Publisher | Elsevier/North Holland Biomedical Press | en_US |
| Rights | restricted access | |
| Title | An imaging flow cytometry-based technique to quantify erythrocyte nuclear alterations | en_US |
| Type | Article | |
| DOI | 10.1016/j.aquatox.2020.105649 | |
| Abstract | Morphological nuclear alterations are indicative of DNA damage and have been considered excellent markers of exposure to several pollutants in aquatic environments. Flow cytometry is a powerful technique for measuring cell phenotypes in large numbers of cells in a short period of time. This technique is suited to the study of cell populations and subset identification as a function of its high-throughput and multi-parameter characteristics. We used the quantification of erythrocyte nuclear alterations to compare the techniques of imaging flow cytometry and light microscopy. The comparison used blood samples of the fish Oreochromis niloticus assayed using cadmium as a nuclear alteration-inducing agent. The results showed that imaging flow cytometry has higher sensitivity than light microscopy for detecting and quantifying erythrocytic nuclear alterations. We conclude that imaging flow cytometry can produce fast and reliable results and could potentially be useful in studies involving fish erythrocytes under normal and impacted environmental conditions. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Department of Morphology. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Department of Pharmacology. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Biochemistry. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Biochemistry. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Department of Morphology. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Oswaldo Cruz Foundation. Instituto Rene Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Department of Morphology. Belo Horizonte, MG, Brazil | en_US |
| Subject | Aquatic environment | en_US |
| Subject | Erythrocytic nuclear alterations | en_US |
| Subject | H2AX | en_US |
| Subject | Imaging flow cytometry | en_US |
| Embargo date | 2099-12-31 | |
