| Autor | Rodrigues, Gisele Regina | |
| Autor | Pinto, Otávio Henrique Bezerra | |
| Autor | Schroeder, Luís Felipe | |
| Autor | Fernandes, Gabriel da Rocha | |
| Autor | Costa, Ohana Yonara Assis | |
| Autor | Quirino, Betania Ferraz | |
| Autor | Kuramae, Eiko Eurya | |
| Autor | Barreto, Cristine Chaves | |
| Data de acesso | 2023-05-24T17:10:59Z | |
| Data de disponibilização | 2023-05-24T17:10:59Z | |
| Data do publicação | 2020 | |
| Citação | RODRIGUES, Gisele Regina et al. Unraveling the xylanolytic potential of Acidobacteria bacterium AB60 from Cerrado soils. FEMS Microbiol Lett., v. 367, n. 18, fnaa149, 2020. doi: 10.1093/femsle/fnaa149. | en_US |
| ISSN | 0378-1097 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58624 | |
| Idioma | eng | en_US |
| Editor | Oxford Oxford University Press | en_US |
| Direito Autoral | restricted access | |
| Título | Unraveling the xylanolytic potential of Acidobacteria bacterium AB60 from Cerrado soils | en_US |
| Tipo do documento | Article | |
| DOI | 10.1093/femsle/fnaa149 | |
| Resumo em Inglês | The presence of genes for glycosyl hydrolases in many Acidobacteria genomes indicates an important role in the degradation of plant cell wall material. Acidobacteria bacterium AB60 was obtained from Cerrado oligotrophic soil in Brazil, where this phylum is abundant. The 16S rRNA gene analyses showed that AB60 was closely related to the genera Occallatibacter and Telmatobacter. However, AB60 grew on xylan as carbon source, which was not observed in Occallatibacter species; but growth was not detected on medium containing carboxymethyl cellulose, as observed in Telmatobacter. Nevertheless, the genome analysis of AB60 revealed genes for the enzymes involved in cellulose as well as xylan degradation. In addition to enzymes involved in xylan degradation, alpha-L-rhamnosidase was detected in the cultures of AB60. Functional screening of a small-insert genomic library did not identify any clones capable of carboxymethyl cellulose degradation, but open reading frames coding alpha-L-arabinofuranosidase and alpha-L-rhamnosidase were present in clones showing xylan degradation halos. Both enzymes act on the lateral chains of heteropolymers such as pectin and some hemicelluloses. These results indicate that the hydrolysis of alpha-linked sugars may offer a metabolic niche for slow-growing Acidobacteria, allowing them to co-exist with other plant-degrading microbes that hydrolyze beta-linked sugars from cellulose or hemicellulose backbones. | en_US |
| Afiliação | Universidade Católica de Brasília. Graduate Program in Genomic Sciences and Biotechnology. Brasília, DF, Brazil. | en_US |
| Afiliação | Universidade Católica de Brasília. Graduate Program in Genomic Sciences and Biotechnology. Brasília, DF, Brazil. | en_US |
| Afiliação | Universidade Católica de Brasília. Graduate Program in Genomic Sciences and Biotechnology. Brasília, DF, Brazil. | en_US |
| Afiliação | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Afiliação | Department of Microbial Ecology. Netherlands Institute of Ecology. Wageningen, The Netherlands. | en_US |
| Afiliação | Brazilian Agricultural Research Corporation. EMBRAPA/Agroenergy. Brasília, DF, Brazil. | en_US |
| Afiliação | Department of Microbial Ecology. Netherlands Institute of Ecology. Wageningen, The Netherlands/Ecology and Biodiversity. Institute of Environmental Biology. Utrecht University. Utrecht, The Netherlands. | en_US |
| Afiliação | Universidade Católica de Brasília. Graduate Program in Genomic Sciences and Biotechnology. Brasília, DF, Brazil. | en_US |
| Palavras-chave em inglês | Acidobacteria | en_US |
| Palavras-chave em inglês | xylanases | en_US |
| Palavras-chave em inglês | cellulases | en_US |
| Palavras-chave em inglês | α-l-rhamnosidase | en_US |
| Palavras-chave em inglês | functional genomics | en_US |
| Palavras-chave em inglês | Cerrado | en_US |
| Data de embargo | 2099-12-31 | |
