| Author | Morawski, Franciele de Matos | |
| Author | Martins, Gustavo | |
| Author | Ramos, Maria Karolina | |
| Author | Zarbin, Aldo J.G. | |
| Author | Blanes, Lucas | |
| Author | Bergamini, Marcio F. | |
| Author | Marcolino-Junior, Luiz Humberto | |
| Access date | 2023-05-24T18:09:18Z | |
| Available date | 2023-05-24T18:09:18Z | |
| Document date | 2023 | |
| Citation | MORAWSKI, Franciele de Matos et al. A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers. Analytica Chimica Acta, v. 1258, p. 1-9, 2023. | en_US |
| ISSN | 0003-2670 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58630 | |
| Language | por | en_US |
| Publisher | Elsevier | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Impressão Tridimensional | en_US |
| Title | A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers | en_US |
| Type | Article | en_US |
| DOI | https://doi.org/10.1016/j.aca.2023.341169 | |
| Abstract | 3D-printing has shown an outstanding performance for the production of versatile electrochemical devices. However, there is a lack of studies in the field of 3D-printed miniaturized settings for multiplex biosensing. In this work, we propose a fully 3D-printed micro-volume cell containing six working electrodes (WEs) that operates with 250 μL of sample. A polylactic acid/carbon black conductive filament (PLA/CB) was used to print the WEs and subsequently modified with graphene oxide (GO), to support protein binding. Cyclic voltammetry was employed to investigate the electrochemical behaviour of the novel multi-electrode cell. In the presence of K₃[Fe (CN)₆], PLA/CB/GO showed adequate peak resolution for subsequent label-free immunosensing. The innovative 3D-printed cell was applied for multiplex voltammetric detection of three COVID-19 biomarkers as a proof-of concept. The multiple sensors showed a wide linear range with detection limits of 5, 1 and 1 pg mL− 1 for Nprotein, SRBD-protein, and anti-SRBD, respectively. The sensor performance enabled the selective sequential detection of N protein, SRBD protein, and anti-SRBD at biological levels in saliva and serum. In summary, the miniaturized six-electrode cell presents an alternative for the low-cost and fast production of customizable devices for multi-target sensing with promising application in the development of point-of-care sensors. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Grupo de Química de Materiais. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Grupo de Química de Materiais. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | en_US |
| Subject | Printing, Three-Dimensional | en_US |
| Subject | Fused Deposition Modelling | en_US |
| Subject | Multiplex Voltammetric Detection | en_US |
| Subject | Biosensing Techniques | en_US |
| Subject in Spanish | Impresión Tridimensional | en_US |
| Subject in Spanish | Técnicas Biosensibles | en_US |
| Subject in French | Impression tridimensionnelle | en_US |
| Subject in French | Techniques de biocapteur | en_US |
| DeCS | Impressão em 3D | en_US |
| DeCS | SARS-CoV-2 | en_US |
| DeCS | Técnicas Biossensoriais | en_US |
