| Author | Nagarkatti, Rana | |
| Author | Acosta, David | |
| Author | Acharyya, Nirmallya | |
| Author | Araujo, Fernanda Fortes de | |
| Author | Santos, Silvana Maria Elói | |
| Author | Martins Filho, Olindo Assis | |
| Author | Carvalho, Andréa Teixeira | |
| Author | Debrabant, Alain | |
| Access date | 2023-05-25T12:10:14Z | |
| Available date | 2023-05-25T12:10:14Z | |
| Document date | 2020 | |
| Citation | NAGARKATT, Rana et al. A novel Trypanosoma cruzi secreted antigen as a potential biomarker of Chagas disease. Sci Rep., v. 10, n. 1, 19591, 2020. doi: 10.1038/s41598-020-76508-1. | en_US |
| ISSN | 2045-2322 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58669 | |
| Sponsorship | U.S. FOOD AND DRUG ADMINISTRATION | en_US |
| Language | eng | en_US |
| Publisher | Nature Publishing Group | en_US |
| Rights | restricted access | |
| Title | A novel Trypanosoma cruzi secreted antigen as a potential biomarker of Chagas disease | en_US |
| Type | Article | |
| DOI | 10.1038/s41598-020-76508-1 | |
| Abstract | Chagas drug discovery has been hampered by a lack of validated assays to establish treatment efficacy in pre-clinical animal models and in patients infected with T. cruzi. Reduced levels of parasite secreted antigens in the blood of infected hosts could be used to demonstrate treatment efficacy. A published proteomic study of parasite secreted antigens identified the hypothetical protein Tc_5171 as a secreted antigen. In this report, we developed Tc_5171 specific antibodies and showed that the native protein was expressed by the three life cycle stages of the parasite. Anti-peptide antibodies were able to detect the parasite antigen in blood of infected mice during the acute and the chronic phase of infection. Benznidazole treatment of infected mice significantly reduced their blood antigen levels. Of clinical significance, patients diagnosed with Chagas disease, either asymptomatic or with cardiac clinical symptoms had significantly higher Tc_5171 antigen levels compared to endemic controls. Pair-wise analysis, before and after Benznidazole treatment, of patients with asymptomatic Chagas disease showed a significant reduction in antigen levels post treatment. Taken together, our results indicate that Tc_5171 could be used as a novel biomarker of Chagas disease for diagnosis and to assess treatment efficacy. | en_US |
| Affilliation | Laboratory of Emerging Pathogens. Division of Emerging and Transfusion Transmitted Diseases. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Affilliation | Laboratory of Emerging Pathogens. Division of Emerging and Transfusion Transmitted Diseases. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Affilliation | Laboratory of Emerging Pathogens. Division of Emerging and Transfusion Transmitted Diseases. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas Em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas Em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Propedêutica Complementar. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas Em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas Em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Laboratory of Emerging Pathogens. Division of Emerging and Transfusion Transmitted Diseases. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Embargo date | 2070-12-31 | |
