| Author | Bandeira, Maria da Conceição Abreu | |
| Author | Rodrigues, Bruno Leite | |
| Author | Soares, Gustavo Henrique Corrêa | |
| Author | Ferreira, André Leite | |
| Author | Costa, Luís Fernando Carvalho | |
| Author | Brazil, Reginaldo Peçanha | |
| Author | Rebêlo, José Manuel Macário | |
| Access date | 2023-05-25T19:33:25Z | |
| Available date | 2023-05-25T19:33:25Z | |
| Document date | 2023 | |
| Citation | BANDEIRA, Maria da Conceição Abreu et al. DNA Barcoding Culicoides Biting Midges (Diptera: Ceratopogonidae) in Northeast Brazil. Journal of Medical Entomology, v. 60, n. 3, p. 608-614, Mar. 2023. | en_US |
| ISSN | 0022-2585 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58730 | |
| Language | eng | en_US |
| Publisher | Oxford University Press | en_US |
| Rights | restricted access | |
| Subject in Portuguese | Taxonomia molecular | en_US |
| Subject in Portuguese | Taxonomia integrativa | en_US |
| Subject in Portuguese | Sistemática molecular | en_US |
| Subject in Portuguese | Mosquitos mordedores | en_US |
| Subject in Portuguese | Delimitação de espécies | en_US |
| Title | DNA Barcoding Culicoides Biting Midges (Diptera: Ceratopogonidae) in Northeast Brazil | en_US |
| Type | Article | |
| DOI | 10.1093/jme/tjad013 | |
| Abstract | Biting midges of the genus Culicoides are small insects associated with the transmission of several pathogens, which
requires the correct identification of the species, for implementation of effective strategies against these insects.
However, many species are difficult to identify only by morphological characters. Therefore, the use of molecular
methods can help in the taxonomy and systematics of this group. Here, the DNA barcode approach was evaluated
for nine species of Culicoides from the State of Maranhão, Brazil. We generated 39 sequences from a 476 bp (base
pairs) fragment of the cytochrome c oxidase subunit I (COI) mitochondrial gene. To assess the usefulness of COI
barcodes for the identification of these species, paired genetic distances from intra and interspecific comparisons
and phylogenetic trees were generated in MEGA and RAxML/BEAST softwares, respectively. In addition, species
delimitation was performed using the PTP, GMYC, and ABGD algorithms. The intra and interspecific genetic
distances showed a clear distinction between them, demonstrating that, for the taxa studied, there can hardly
be ambiguous identifications with barcodes. In the same sense, the phylogenetic reconstruction resulted in wellsupported clades for all morphospecies analyzed. | en_US |
| Affilliation | Universidade Federal do Maranhão. Laboratório de Entomologia e Vetores. São Luís, Maranhão, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de PósGraduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade de São Paulo, Faculdade de Saúde Pública, Programa de Pós-Graduação em Saúde Pública. São Paulo, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Maranhão. Laboratório de Entomologia e Vetores. São Luís, Maranhão, Brasil. | en_US |
| Affilliation | Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Biologia. São Luís, MA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Maranhão. Laboratório de Entomologia e Vetores. São Luís, Maranhão, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Molecular taxonomy | en_US |
| Subject | Integrative taxonomy | en_US |
| Subject | Molecular systematics | en_US |
| Subject | Biting midges | en_US |
| Subject | Species delimitation | en_US |
| Embargo date | 2028 | |
