| Author | Melo, Éricka Lorenna de Sales Souza e | |
| Author | Campos, Caroline Leonel Vasconcelos de | |
| Author | Reis, Diego Carlos dos | |
| Author | Cassali, Geovanni Dantas | |
| Author | Generoso, Simone de Vasconcelos | |
| Author | Cardoso, Valbert Nascimento | |
| Author | Azevedo, Vasco | |
| Author | Medeiros, Julliane Dutra | |
| Author | Fernandes, Gabriel da Rocha | |
| Author | Jacques Robert Nicoli | |
| Author | Flaviano dos Santos Martins | |
| Access date | 2023-05-26T14:34:51Z | |
| Available date | 2023-05-26T14:34:51Z | |
| Document date | 2020 | |
| Citation | SOUZA, Éricka Lorenna de Sales et al. Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model. Beneficial Microbes, v. 11, n. 8, 2020. doi.org/10.3920/BM2020.0045 | en_US |
| ISSN | 1876-2883 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58745 | |
| Language | eng | en_US |
| Publisher | Wageningen Academic Publishers | en_US |
| Rights | restricted access | |
| Title | Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model | en_US |
| Type | Article | |
| DOI | 10.3920/BM2020.0045 | |
| Abstract | Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals. Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC). Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation. The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germfree (GF) and conventional (CV) mice. CV female mice were used for clinical, immunological and permeability experiments. GF mice were used for a faecal microbiota transplantation assay. To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals. For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log(10) cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment. EcN presented beneficial effects when administered preventively. Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one. Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier. Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-gamma levels were observed in EcN treatment. Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups. After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota. This was observed indirectly in the reduced daily DAI, when colitis was compared with treated group. In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC. | en_US |
| Affilliation | Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of General Pathology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of General Pathology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Nutrition. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Clinical and Toxicological Analysis. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Genetic, Ecology and Evolution. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | |
| Affilliation | Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | |
| Subject | Escherichia coli Nissle 1917 | en_US |
| Subject | inflammatory bowel disease | en_US |
| Subject | intestinal microbiota | en_US |
| Subject | probiotic | en_US |
| Subject | ulcerative colitis | en_US |
| Embargo date | 2099-12-31 | |
