| Author | Oliveira, Edward José de | |
| Author | Pedras, Mariana Junqueira | |
| Author | Assis, Isabella Elias Morais de | |
| Author | Rabello, Ana Lucia Teles | |
| Access date | 2023-05-29T18:01:37Z | |
| Available date | 2023-05-29T18:01:37Z | |
| Document date | 2009 | |
| Citation | OLIVEIRA, Edward José de et al. Improvement of direct agglutination test (DAT) for laboratory diagnosis of visceral leishmaniasis in Brazil. Trans R Soc Trop Med Hyg., v. 103, n. 12, p. 1279-1281, 2009. doi: 10.1016/j.trstmh.2009.04.007 | en_US |
| ISSN | 0035-9203 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/58804 | |
| Language | eng | en_US |
| Publisher | Oxford University Press | en_US |
| Rights | restricted access | |
| Title | Improvement of direct agglutination test (DAT) for laboratory diagnosis of visceral leishmaniasis in Brazil | en_US |
| Type | Article | |
| DOI | 10.1016/j.trstmh.2009.04.007 | |
| Abstract | We previously standardized the direct agglutination test (DAT) to detect antiLeishmania chagasi promastigote antibodies (DAT-LPC) with good sensitivity and specificity for diagnosing visceral. leishmaniasis (VL). In this paper, we present a technical upgrade by introducing some modifications into the antigen preparation. This antigen was evaluated in DAT (DAT-Mod) using 61 sera samples from VL patients and 96 samples from patients with other diseases. The DAT-Mod presented a cut-off of 1:100, satisfactory reproducibility (VC <5.8), sensitivity of 93.4%, specificity of 96.9%, and diagnostic efficiency of 95.5%. The improvement in antigen preparation reduced inter-batch variations and resulted in a high test performance. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Visceral leishmaniasis | en_US |
| Subject | Serologic test | en_US |
| Subject | Diagnosis | en_US |
| Subject | DAT | en_US |
| Subject | Sensitivity and specificity | en_US |
| Subject | Brazil | en_US |
| Embargo date | 2099-12-31 | |
