| Author | Soares, Rodrigo Pedro Pinto | |
| Author | Margonari, Carina | |
| Author | Secundino, Nágila Francinete Costa | |
| Author | Macêdo, Maria E. | |
| Author | Costa, Simone M. da | |
| Author | Rangel, Elizabeth F. | |
| Author | Pimenta, Paulo Filemon Paolucci | |
| Author | Turco, Salvatore Joseph | |
| Access date | 2012-11-27T17:01:02Z | |
| Available date | 2012-11-27T17:01:02Z | |
| Document date | 2010 | |
| Citation | SOARES, Rodrigo Pedro Pinto et al. DifferentialMidgut Attachment of Leishmania (Viannia) braziliensis in the Sand Flies Lutzomyia (Nyssomyia) whitmani and Lutzomyia (Nyssomyia) intermédia. Journal of Biomedicine and Biotechnology, v. 2010, p. 1-7, 2010. | pt_BR |
| ISSN | 1110-7243 | |
| URI | https://www.arca.fiocruz.br/handle/icict/5881 | |
| Sponsorship | National Council for the Development of Research of Brazil (CNPq), Special Programme for Research and Training in Tropical Diseases (TDR), National Institutes of Health | pt_BR |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | |
| Title | Differential Midgut Attachment of Leishmania (Viannia) braziliensis in the Sand Flies Lutzomyia (Nyssomyia) whitmani and Lutzomyia (Nyssomyia) intermedia | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2010/439174 | |
| Abstract | The interaction between Leishmania and sand flies has been demonstrated in many Old and New World species. Besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (LPG). An upregulation of β-glucose residues was previously shown in the LPG repeat units from procyclic to metacyclic phase in Leishmania (Viannia) braziliensis, which has not been reported in any Leishmania species. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly in the Subgenus Leishmania. These adaptations were explored for the first time in a species fromthe Subgenus Viannia, L. (V.) braziliensis with its natural vectors Lutzomyia (Nyssomyia) intermedia and Lutzomyia (Nyssomyia) whitmani. Using two in vitro binding techniques, phosphoglycans (PGs) derived from procyclic and metacyclic parasites were able to bind to the insect midgut and inhibit L. braziliensis attachment. Interestingly, L. braziliensis procyclic parasite attachment was ∼11-fold greater in the midgut of L. whitmani than in L. intermedia. The epidemiological relevance of L. whitmani as a vector of American Cutaneous Leishmaniasis | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | University of Kentucky Medical Center. College of Medicine. Department of Biochemistry Lexington, KY, USA. | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
