| Author | Bayona-Serrano, J. D. | |
| Author | Grazziotin, F. G. | |
| Author | Salazar-Valenzuela, D. | |
| Author | Valente, R. H. | |
| Author | Nachtigall, P. G. | |
| Author | Colombini, M. | |
| Author | Moura da Silva, A. M. | |
| Author | Junqueira-de-Azevedo, I. L. M. | |
| Access date | 2023-06-26T18:03:14Z | |
| Available date | 2023-06-26T18:03:14Z | |
| Document date | 2023 | |
| Citation | BAYONA-SERRANO, J. D. et al. Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae). | |
| URI | https://www.arca.fiocruz.br/handle/icict/59182 | |
| Description | Running title: Evolution of the PLA2 scaffold within rear-fanged snakes | en_US |
| Language | eng | en_US |
| Publisher | Oxford University Press | |
| Rights | open access | |
| Subject in Portuguese | Fosfolipases A2 | pt_BR |
| Subject in Portuguese | Evolução da família de proteínas | pt_BR |
| Subject in Portuguese | Cooptação ene | pt_BR |
| Subject in Portuguese | Veneno de cobra | pt_BR |
| Subject in Portuguese | Dipsadidae | pt_BR |
| Title | Independent recruitment of different types of phospholipases A2 to the venoms of Caenophidian snakes: the rise of PLA2-IIE within Pseudoboini (Dipsadidae) | en_US |
| Type | Preprint | |
| Abstract | Snake venom harbors a wide and diverse array of enzymatic and nonenzymatic toxic 2 components, allowing them to exert myriad effects on their prey. However, they appear to 3 trend toward a few optimal compositional scaffolds, dominated by four major toxin 4 classes: SVMPs, SVSPs, 3FTxs and PLA2s. Nevertheless, the latter appears to be 5 restricted to vipers and elapids, as it has never been reported as a major venom component 6 in rear-fanged species. Here, by investigating the original transcriptomes from 19 species 7 distributed in eight genera from the Pseudoboini tribe (Dipsadidae: Xenodontinae) and 8 screening among seven additional tribes of Dipsadidae and three additional families of 9 advanced snakes, we discovered that a novel type of venom, PLA2, resembling a PLA2-10 IIE, has been recruited to the venom of some species of the Pseudoboini tribe, where it is a major component. Proteomic and functional analyses of these venoms further indicate 12 that these PLA2s play a relevant role in the venoms from this tribe. Moreover, we 13 reconstructed the phylogeny of PLA2s across different snake groups and show that 14 different types of these toxins have been recruited in at least five independent events in 15 caenophidian snakes. Additionally, we present the first compositional profiling of 16 Pseudoboini venoms. Our results demonstrate how relevant phenotypic traits are 17 convergently recruited by different means and from homologous and nonhomologous 18 genes in phylogenetically and ecologically divergent snake groups, possibly optimizing venom composition to overcome diverse adaptative landscapes. | en_US |
| Affilliation | Instituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil. | |
| Affilliation | Instituto Butantan. Laboratório de Coleções Zoológicas (LECZ). São Paulo, SP, Brasil. | |
| Affilliation | Universidad Indoamérica, Machala y Sabanilla 170301. Facultad de Ciencias del Medio Ambiente. Ingeniería en Biodiversidad y Recursos Genéticos. Centro de Investigación de la Biodiversidad y Cambio Climático (BioCamb). Quito, Ecuador. | |
| Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Instituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil. | |
| Affilliation | Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil. | |
| Affilliation | Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil. | |
| Affilliation | Instituto Butantan. Laboratório de Toxinologia Aplicada (LETA), São Paulo, SP, Brasil / Center of Toxins, Immune-Response and Cell Signaling (CeTICS), São Paulo, SP, Brazil. | |
| Subject | Phospholipases A2 | en_US |
| Subject | Protein family evolution | en_US |
| Subject | Gene co-option | en_US |
| Subject | Snake venom | en_US |
| Subject | Dipsadidae | en_US |
