Author | Queiroz, Rafaella Fortini Grenfell e | |
Author | Harn, Donald A. | |
Author | Tundup, Smanla | |
Author | Da'dara, Akram | |
Author | Siqueira, Liliane | |
Author | Coelho, Paulo Marcos Zech | |
Access date | 2023-06-30T17:54:37Z | |
Available date | 2023-06-30T17:54:37Z | |
Document date | 2013 | |
Citation | QUEIROZ, Rafaella Fortini Grenfell e et al. New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. PLoS Negl Trop Dis., v. 7, n. 2, e2054, 2013. doi: 10.1371/journal.pntd.0002054. | en_US |
ISSN | 1935-2735 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59322 | |
Language | eng | en_US |
Publisher | Public Library of Science | en_US |
Rights | open access | en_US |
Title | New Approaches with Different Types of Circulating Cathodic Antigen for the Diagnosis of Patients with Low Schistosoma mansoni Load | en_US |
Type | Article | en_US |
DOI | 10.1371/journal.pntd.0002054 | |
Abstract | Background: Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens. Method: Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as "crude antigen," the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen-antibody binding. Principal Findings: Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the "crude antigen" worked as a good marker for control of cure after praziquantel treatment. Conclusions/Significance: Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patients | en_US |
Affilliation | Schistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of America | en_US |
Affilliation | Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of America | en_US |
Affilliation | Department of Infectious Diseases. College of Veterinary Medicine and the Center for Tropical and Emerging Diseases. University of Georgia. Athens, GA, United States of America | en_US |
Affilliation | Tufts University. Grafton, MA, United States of America/Centers for Disease Control and Prevention. United States of America | en_US |
Affilliation | Schistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Schistosomiasis Laboratory. Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | en_US |