| Author | Steindel, Mario | |
| Author | Pacheco, Letícia Kramer | |
| Author | Scholl, Daniele | |
| Author | Soares, Marcos | |
| Author | Moraes, Milene Hoehr de | |
| Author | Eger, Iriane | |
| Author | Kosmann, Cecília | |
| Author | Sincero, Thais Cristine Marques | |
| Author | Stoco, Patrícia Hermes | |
| Author | Murta, Silvane Maria Fonseca | |
| Author | Carvalho-Pinto, Carlos José de | |
| Author | Grisard, Edmundo Carlos | |
| Access date | 2023-06-30T19:12:46Z | |
| Available date | 2023-06-30T19:12:46Z | |
| Document date | 2008 | |
| Citation | STEINDEL, Mário et al. Characterization of Trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human Chagas disease in Santa Catarina State, Brazil. Diagn Microbiol Infect Dis., v. 60, n. 1, p. 25-32, 2008. doi: 10.1016/j.diagmicrobio.2007.07.016. | en_US |
| ISSN | 0732-8893 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59332 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Title | Characterization of Trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human Chagas disease in Santa Catarina State, Brazil | en_US |
| Type | Article | |
| DOI | 10.1016/j.diagmicrobio.2007.07.016 | |
| Abstract | During March 2005, 24 cases of acute human Chagas disease were detected in Santa Catarina State, southern Brazil, all of them related to the ingestion of Trypanosoma cruzi-contaminated sugar cane juice. Following field studies allowed the isolation of 13 T cruzi strains from humans, opossums (Didelphis aurita and Didelphis albiventris), and vectors (Triatoma tibiamaculata). The isolated strains were characterized by multilocus enzyme electrophoresis (MLEE) and analysis of the spliced-leader and 24S alpha rRNA genes. The assays revealed that all strains isolated from humans belong to the Tell group but revealed a TcII variant pattern for the phosphoglucomutase enzyme. Strains isolated from opossums also showed a TcI profile in all analysis, but strains isolated from triatomines revealed a mixed TcI/TcII profile by MLEE. No indication of the presence of Trypanosoma rangeli was observed in any assay. Considering that mixed strains (TcI/TcII) were isolated from triatomines in an area without active vectorial transmission to humans and that all strains isolated from humans belong to the Tell group, our results show that T cruzi TcI and Tell groups are circulating among reservoirs and vectors in southern Brazil and indicate that selection toward Tell group in humans may occur after ingestion of a mixed (TcI/TeII) T cruzi population. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil/Universidade do Vale do Itajaí. Itajaí, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brazil | en_US |
| Subject | human Chagas disease | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | oral infection | en_US |
| Subject | MLEE | en_US |
| Subject | strain characterization | en_US |
| Subject | PCR | en_US |
| Embargo date | 2099-12-31 | |
