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| Autor | Macedo, Cristiana Santos de | |
| Autor | Anderson, David M. | |
| Autor | Schey, Kevin L. | |
| Fecha de acceso | 2023-07-03T20:49:30Z | |
| Fecha de disponibilización | 2023-07-03T20:49:30Z | |
| Fecha de publicación | 2017 | |
| Referencia | MACEDO, Cristiana Santos de; ANDERSON, David M.; SCHEY, Kevin L. MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) of skin: Aspects of sample preparation. Talanta, Netherlands, v. 174, p. 325-335, Nov. 2017. | en_US |
| ISSN | 0039-9140 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59379 | |
| Idioma | eng | en_US |
| Editor | Elsevier | en_US |
| Derechos de autor | restricted access | |
| Título | MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) of skin: Aspects of sample preparation | en_US |
| Tipo del documento | Article | |
| DOI | 10.1016/J.TALANTA.2017.06.018 | |
| Resumen en Inglés | MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) allows molecular analysis of biological materials making possible the identification and localization of molecules in tissues, and has been applied to address many questions on skin pathophysiology, as well as on studies about drug absorption and metabolism. Sample preparation for MALDI IMS is the most important part of the workflow, comprising specimen collection and preservation, tissue embedding, cryosectioning, washing, and matrix application. These steps must be carefully optimized for specific analytes of interest (lipids, proteins, drugs, etc.), representing a challenge for skin analysis. In this review, critical parameters for MALDI IMS sample preparation of skin samples will be described. In addition, specific applications of MALDI IMS of skin samples will be presented including wound healing, neoplasia, and infection. | en_US |
| Afiliación | Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brazil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Cellular Microbiology Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
| Afiliación | Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA. | en_US |
| Afiliación | Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA. | en_US |
| Palavras clave en Inglês | Maldi matrices | en_US |
| Palavras clave en Inglês | Molecular histology | en_US |
| Palavras clave en Inglês | Pathophysiology | en_US |
| Palavras clave en Inglês | Direct tissue-analysis | en_US |
| Palavras clave en Inglês | Spatial-resolution | en_US |
| Palavras clave en Inglês | Lipid changes | en_US |
| Palavras clave en Inglês | Formalin | en_US |
| Palavras clave en Inglês | MSI | en_US |
| Palavras clave en Inglês | Quantitation | en_US |
| Palavras clave en Inglês | Sensitivity | en_US |
| Palavras clave en Inglês | Metabolites | en_US |
| Palavras clave en Inglês | Absorption | en_US |
| Palavras clave en Inglês | Sections | en_US |
| e-ISSN | 1873-3573 | |
| Fecha de embargo | 2030-12-31 |
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