| Author | Ribeiro, Thales de P. | |
| Author | Fonseca, Fernanda L. | |
| Author | Carvalho, Mariana D. C. de | |
| Author | Godinho, Rodrigo M. da C. | |
| Author | Almeida, Fernando Pereira de | |
| Author | Saint'Pierre, Tatiana D. | |
| Author | Rey, Nicolás A. | |
| Author | Fernandes, Christiane | |
| Author | Horn, Adolfo | |
| Author | Pereira, Marcos D. | |
| Access date | 2023-07-03T22:00:32Z | |
| Available date | 2023-07-03T22:00:32Z | |
| Document date | 2017 | |
| Citation | RIBEIRO, Thales de P. et al. Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae. Biochemical Journal, v. 474, n. 2, p. 301-315, Jan. 2017. | en_US |
| ISSN | 0264-6021 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/59383 | |
| Language | eng | en_US |
| Publisher | Portland Press Ltd | en_US |
| Rights | restricted access | |
| MeSH | Biomarkers | en_US |
| MeSH | Biomimetic Materials | en_US |
| MeSH | Catalase | en_US |
| MeSH | Coordination Complexes | en_US |
| MeSH | Copper | en_US |
| MeSH | Hydrogen Peroxide | en_US |
| MeSH | Iron | en_US |
| MeSH | Lipid Droplets | en_US |
| MeSH | Manganese | en_US |
| MeSH | Microbial Viability | en_US |
| MeSH | Oxidation-Reduction | en_US |
| MeSH | Oxidative Stress | en_US |
| MeSH | Pyridines | en_US |
| MeSH | Saccharomyces cerevisiae | en_US |
| MeSH | Saccharomyces cerevisiae Proteins | en_US |
| MeSH | Superoxide Dismutase | en_US |
| Title | Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae | en_US |
| Type | Article | |
| DOI | 10.1042/BCJ20160480 | |
| Abstract | Aging is a natural process characterized by several biological changes. In this context, oxidative stress appears as a key factor that leads cells and organisms to severe dysfunctions and diseases. To cope with reactive oxygen species and oxidative-related damage, there has been increased use of superoxide dismutase (SOD)/catalase (CAT) biomimetic compounds. Recently, we have shown that three metal-based compounds {[Fe(HPClNOL)Cl2]NO3, [Cu(HPClNOL)(CH3CN)](ClO4)2 and Mn(HPClNOL)(Cl)2}, harboring in vitro SOD and/or CAT activities, were critical for protection of yeast cells against oxidative stress. In this work, treating Saccharomyces cerevisiae with these SOD/CAT mimics (25.0 µM/1 h), we highlight the pivotal role of these compounds to extend the life span of yeast during chronological aging. Evaluating lipid and protein oxidation of aged cells, it becomes evident that these mimics extend the life expectancy of yeast mainly due to the reduction in oxidative stress biomarkers. In addition, the treatment of yeast cells with these mimics regulated the amounts of lipid droplet occurrence, consistent with the requirement and protection of lipids for cell integrity during aging. Concerning SOD/CAT mimics uptake, using inductively coupled plasma mass spectrometry, we add new evidence that these complexes, besides being bioabsorbed by S. cerevisiae cells, can also affect metal homeostasis. Finally, our work presents a new application for these SOD/CAT mimics, which demonstrate a great potential to be employed as antiaging agents. Taken together, these promising results prompt future studies concerning the relevance of administration of these molecules against the emerging aging-related diseases such as Parkinson's, Alzheimer's and Huntington's. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Citotoxicidade e Genotoxicidade. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Citotoxicidade e Genotoxicidade. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Laboratório de Espectrometria Atômica. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Laboratório de Síntese Orgânica e Química de Coordenação Aplicada a Sistemas Biológicos. Rio de Janeiro, Brazil. | en_US |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Ciências Químicas. Campos dos Goytacazes, RJ, Brazil. | en_US |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Ciências Químicas. Campos dos Goytacazes, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Citotoxicidade e Genotoxicidade. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Saccharomyces cerevisiae | en_US |
| Subject | Superoxide | en_US |
| Subject | Biomarkers | en_US |
| Subject | Metal | en_US |
| Subject | SOD/CAT | en_US |
| Subject | Parkinson | en_US |
| Subject | Alzheimer | en_US |
| Subject | Huntington | en_US |
| Subject | Neurodegeneration | en_US |
| Subject | Metal homeostasis | en_US |
| Subject | Manganese complexes | en_US |
| Subject | Overexpression | en_US |
| Subject | Neutral lipid storage | en_US |
| e-ISSN | 1470-8728 | |
| Embargo date | 2030-12-31 | |
